Medium composition suitable for L-lysine production by Methylophilus methylotrophus in fed-batch cultivation

Journal of Bioscience and Bioengineering
Kohei IshikawaKozo Asano

Abstract

L-Lysine production was investigated in fed-batch fermentation using L-lysine producer of Methylophilus methylotrophus. By the addition of nutrient composition, containing L-methionine, K(2)HPO(4), NaH(2)PO(4), CuSO(4).5aq, MnSO(4).5aq, ZnSO(4).7aq, FeCl(3), MgSO(4).7aq and CaCl(2).2aq, in the feed medium, cell growth could be maintained through the cultivation, and L-lysine production reached to 7.86 g. In addition, the effect of counter ion for NH(4)(+) (Cl(-), SO(4)(2-), glutamate, succinate and citrate) was examined. The result showed that the cell growth in the medium using Cl(-) and glutamate were improved compared with that using SO(4)(2-), succinate and citrate, and L-lysine production in the medium using Cl(-) and glutamate reached to more than 9.0 g. In this experiment, there was a clear correlation between ionic strength and growth rate in the cultivation. In order to examine the influence of ionic strength on growth rate, the activity of enzymes in central metabolic pathway from methanol to pyruvate were assayed using samples at the log-phase and the stationary phase in fed-batch cultivation using (NH(4))(2)SO(4) and (NH(4))Cl as ammonium source. It was found that the higher ionic strength inhibited methanol oxidati...Continue Reading

References

May 1, 1995·Microbiology·Pat M Goodwin, Christopher Anthony
May 29, 1999·Applied and Environmental Microbiology·M Ikeda, R Katsumata
Sep 2, 2003·Journal of Biotechnology·Thomas Hermann
Jul 28, 2004·Bioscience, Biotechnology, and Biochemistry·Yoshiya GunjiHisashi Yasueda
Feb 18, 2006·Journal of Biotechnology·Nobuharu TsujimotoHisashi Yasueda
May 8, 2008·Bioscience, Biotechnology, and Biochemistry·Kohei IshikawaKozo Asano

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