Medroxyprogesterone acetate drives M2 macrophage differentiation toward a phenotype of decidual macrophage

Molecular and Cellular Endocrinology
Yung-Chieh TsaiPao-Lin Kuo

Abstract

M1 macrophage differentiation plays a crucial role in enhanced inflammation during pregnancy, which may lead to pregnancy complications. Therefore, modulation of macrophage differentiation toward the M2 phenotype is desirable to ensure a successful pregnancy. Medroxyprogesterone acetate (MPA) is a potent progestin with an anti-inflammatory property, but its effect on macrophage differentiation is unknown. This study aimed to examine whether MPA can induce an M2 macrophage differentiation by using the human monocytes cell line THP-1 or primary monocytes. THP-1 cells were primed with phorbol-12-myristate-13 acetate (PMA) to initiate macrophage differentiation. By incubating with MPA, the cells (denoted as MPA-pTHP-1) underwent M2 macrophage differentiation with downregulations of CD11c, IL-1β and TNF-α, and upregulations of CD163 and IL-10; while cells incubated with progesterone (P4) did not show the M2 phenotype. Primary monocytes treated with MPA also had the same M2 phenotype. Moreover, M1 macrophages derived from IFN-γ/LPS-treated THP-1 cells, which had high levels of IL-1b and iNOS, and low levels of IL-10 and IDO, were reversed to the M2 phenotype by the MPA treatment. We also found that the MPA-pTHP-1 promoted the decidua...Continue Reading

Citations

Mar 4, 2018·American Journal of Reproductive Immunology : AJRI·Yan-Ran ShengXiao-Yong Zhu
Apr 16, 2019·Frontiers in Endocrinology·Nishel M ShahMark R Johnson
Feb 11, 2020·Frontiers in Immunology·Maria Emilia Solano, Petra Clara Arck
Mar 18, 2020·Frontiers in Immunology·Li-Na MaAi-Hua Liao
Mar 21, 2020·Frontiers in Immunology·Yi-Shu HuangTrevor W Stone

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