Medroxyprogesterone acetate inhibits proliferation of colon cancer cell lines by modulating cell cycle-related protein expression

Menopause : the Journal of the North American Menopause Society
Yoshihiro TanakaNorio Wake

Abstract

To investigate the effects of medroxyprogesterone acetate on colon cancer cells in vitro. HT29 and HCT116 human colon cancer cell lines were used in this study. Cell growth and WST-1 assays were performed to investigate the antiproliferative effect of medroxyprogesterone acetate. Cell cycle analysis was performed to investigate the effects of medroxyprogesterone acetate on cell cycle distribution. Western blot, immunoprecipitation, and a cyclin-dependent kinase assay were performed to investigate changes in the levels of cell cycle proteins. Medroxyprogesterone acetate inhibited proliferation of the cancer cells by inducing accumulation in the G0/G1 fraction. Medroxyprogesterone acetate decreased expression of cyclin E, increased expression of p21(WAF1/CIP1), and enhanced interaction of p21(WAF1/CIP1) with cyclin-dependent kinase 2, eventually inhibiting its activity. Medroxyprogesterone acetate exerts its antiproliferative effect by modulating cell cycle-related protein expression and cyclin-dependent kinase 2 activity. These results should help to elucidate the protective effect of medroxyprogesterone acetate on colon cancer risk.

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Citations

Apr 9, 2008·Menopause : the Journal of the North American Menopause Society·Richard T Penson
Oct 31, 2009·Journal of Gastroenterology and Hepatology·Jenn Hian Koo, Rupert W L Leong
Aug 6, 2011·PloS One·Jarom HeijmansGijs R van den Brink
Mar 5, 2009·Climacteric : the Journal of the International Menopause Society·Anna Fenton, Nick Panay
Feb 10, 2019·Endocrine Connections·Corina Verónica SassoRubén Walter Carón
Jun 13, 2009·Menopause : the Journal of the North American Menopause Society·John F Donaldson
Jun 15, 2014·Experimental Biology and Medicine·Dong-Qing WangZhen-Hai Yu
Jun 16, 2019·Clinical Epigenetics·Cheng LiuVicki L J Whitehall
Aug 24, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Afsaneh BarziHeinz-Josef Lenz

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