Meeting report: GARNet/OpenPlant CRISPR-Cas workshop

Plant Methods
Geraint ParryColette Matthewman

Abstract

Targeted genome engineering has been described as a "game-changing technology" for fields as diverse as human genetics and plant biotechnology. One technique used for precise gene editing utilises the CRISPR-Cas system and is an effective method for genetic engineering in a wide variety of plants. However, many researchers remain unaware of both the technical challenges that emerge when using this technique or of its potential benefits. Therefore in September 2015, GARNet and OpenPlant organized a two-day workshop at the John Innes Centre that provided both background information and hands-on training for this important technology.

References

Jun 11, 2010·Public Understanding of Science·Martha AugoustinosRichard Shepherd
Dec 1, 2011·Bioengineered Bugs·Stefan WernerSylvestre Marillonnet
Jun 12, 2013·PloS One·Matin Qaim, Shahzad Kouser
Aug 10, 2013·Nature Biotechnology·Vladimir NekrasovSophien Kamoun
Dec 18, 2013·Science·Tim WangEric S Lander
May 20, 2014·The Plant Journal : for Cell and Molecular Biology·Friedrich FauserHolger Puchta
Jun 17, 2014·ACS Synthetic Biology·Carola EnglerSylvestre Marillonnet
Dec 24, 2014·Biotechnology Advances·Luisa Bortesi, Rainer Fischer
Jul 15, 2015·The New Phytologist·Nicola J PatronJim Haseloff

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Citations

Aug 1, 2020·Journal of Experimental Botany·Panupon KhumsupanAlistair J McCormick

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Methods Mentioned

BETA
targeted mutations
targeted
transgenic
PCR
transfection

Software Mentioned

MoClo
GARNet
Guidebook
OpenPlant
Desktop Genetics

Related Concepts

Related Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

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