DOI: 10.1101/473215Nov 20, 2018Paper

Meeting the challenges of high-dimensional data analysis in immunology

BioRxiv : the Preprint Server for Biology
Christina ZielinskiSubarna Palit

Abstract

Recent advances in cytometry have radically altered the fate of single-cell proteomics by allowing a more accurate understanding of complex biological systems. Mass cytometry (CyTOF) provides simultaneous single-cell measurements that are crucial to understand cellular heterogeneity and identify novel cellular subsets. High-dimensional CyTOF data were traditionally analyzed by gating on bivariate dot plots, which are not only laborious given the quadratic increase of complexity with dimension but are also biased through manual gating. This review aims to discuss the impact of new analysis techniques for in-depths insights into the dynamics of immune regulation obtained from static snapshot data and to provide tools to immunologists to address the high dimensionality of their single-cell data.

Related Concepts

T-Lymphocyte Subsets
Proteome
Cytometry
Analysis
Regulation of Immune Response
2-Dimensional

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