MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a

Molecular Medicine Reports
Shibao ZhangZhonghua Xu

Abstract

Prostate cancer gene expression marker 1 (PCGEM1) is a prostate‑specific gene overexpressed in prostate cancer cells that promotes cell proliferation. To study the molecular mechanism of PCGEM1 function in hormone‑refractory prostate cancer, the interaction between myocyte enhancer factor 2 (MEF2) and PCGEM1 was assessed by a luciferase reporter assay and chromatin immunoprecipitation (ChIP) assay. In addition, the underlying mechanism of PCGEM1 regulating expression of microRNA (miR)‑148a in PC3 prostate cancer cells was evaluated. Relative expression levels were measured by reverse transcription‑quantitative polymerase chain reaction, and early apoptosis was measured by flow cytometry. PCGEM1 was demonstrated to be overexpressed in prostate cancer tissues compared with noncancerous tissues. Expression levels of PCGEM1 in PC3 cancer cells were demonstrated to be regulated by MEF2, as PCGME1 mRNA was increased by MEF2 overexpression but decreased by MEF2 silencing. MEF2 was also demonstrated to enhance the activity of PCGEM1 promoter and thus promote PCGEM1 transcription. In addition, downregulation of PCGEM1 expression in PC3 cells increased expression of miR‑148a. By contrast, overexpression of PCGEM1 decreased miR‑148a expre...Continue Reading

References

Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·V SrikantanS Srivastava
Feb 14, 2002·Trends in Biochemical Sciences·Timothy A McKinseyEric N Olson
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 25, 2006·Cancer Genetics and Cytogenetics·Cláudia M Coutinho-CamilloMaria A Nagai
Apr 20, 2010·Journal of Molecular and Cellular Cardiology·Annett SchroeterThomas Zimmer
Jan 12, 2011·Nature Biotechnology·James T RobinsonJill P Mesirov
Apr 2, 2011·Cell Death and Differentiation·H ZhangM Lai
Apr 21, 2012·Briefings in Bioinformatics·Helga ThorvaldsdóttirJill P Mesirov
Feb 14, 2013·PloS One·Saakshi JalaliVinod Scaria
Apr 27, 2013·Biology Direct·Saurabh V LaddhaArijit Mukhopadhyay
Jun 12, 2013·The International Journal of Biochemistry & Cell Biology·Chi Han Li, Yangchao Chen
Aug 27, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Rui ZhangLi Li
Apr 4, 2014·Molecular Endocrinology·Caroline DaemsJacques J Tremblay
Jun 14, 2014·Brain Tumor Research and Treatment·Ju Young ParkJong Heon Kim
Sep 10, 2014·Journal of Experimental & Clinical Cancer Research : CR·Jin-Hua HeYu-Guang Li
Nov 28, 2014·Oncology Research and Treatment·Zhi ChenXiang Chen
Sep 30, 2016·Scientific Reports·Tsui-Ting HoYin-Yuan Mo
Nov 30, 2016·Genes & Development·Natalia SacilottoSarah De Val

❮ Previous
Next ❯

Citations

Jan 29, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·J ZhangY Zhao

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
immunoprecipitation assay
protein assay
X-ray
transfection
immunoprecipitation
PCR
flow cytometry
ChIP

Software Mentioned

DPS
RegRNA
Quantity One
GraphPad
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.