Mef2c-F10N enhancer driven β-galactosidase (LacZ) and Cre recombinase mice facilitate analyses of gene function and lineage fate in neural crest cells

Developmental Biology
Kazushi AotoPaul A Trainor

Abstract

Neural crest cells (NCC) comprise a multipotent, migratory stem cell and progenitor population that gives rise to numerous cell and tissue types within a developing embryo, including craniofacial bone and cartilage, neurons and glia of the peripheral nervous system, and melanocytes within the skin. Here we describe two novel stable transgenic mouse lines suitable for lineage tracing and analysis of gene function in NCC. Firstly, using the F10N enhancer of the Mef2c gene (Mef2c-F10N) linked to LacZ, we generated transgenic mice (Mef2c-F10N-LacZ) that express LacZ in the majority, if not all migrating NCC that delaminate from the neural tube. Mef2c-F10N-LacZ then continues to be expressed primarily in neurogenic, gliogenic and melanocytic NCC and their derivatives, but not in ectomesenchymal derivatives. Secondly, we used the same Mef2c-F10N enhancer together with Cre recombinase to generate transgenic mice (Mef2c-F10N-Cre) that can be used to indelibly label, or alter gene function in, migrating NCC and their derivatives. At early stages of development, Mef2c-F10N-LacZ and Mef2c-F10N-Cre label NCC in a pattern similar to Wnt1-Cre mice, with the exception that Mef2c-F10N-LacZ and Mef2c-F10N-Cre specifically label NCC that have de...Continue Reading

References

Mar 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J S Collins, T H Goldsmith
Apr 1, 1983·The American Journal of Anatomy·A D'Amico-Martel, D M Noden
Apr 1, 1994·Anatomy and Embryology·P P Tam, P A Trainor
Mar 21, 2000·Genesis : the Journal of Genetics and Development·J LiJ A Epstein
Jan 11, 2002·Developmental Biology·Xiaobing JiangGillian M Morriss-Kay
Dec 11, 2002·The Journal of Cell Biology·Lisette HariLukas Sommer
Nov 5, 2003·Genesis : the Journal of Genetics and Development·Ian D TonksGraham F Kay
Jul 22, 2005·Nature·Toshiyuki MatsuokaGeorgy Koentges
Dec 13, 2005·Journal of Cell Science·Martin H M SailerRonald D G McKay
Apr 11, 2007·Developmental Cell·Michael P VerziBrian L Black
Oct 26, 2007·Development·Matthew J Potthoff, Eric N Olson
Jul 12, 2008·Mechanisms of Development·Toshiyuki YoshidaSachiko Iseki
Jul 16, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Barbara MurdochMartín I García-Castro
Nov 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Perrine BarraudClare V H Baker
Jan 22, 2011·Current Opinion in Genetics & Development·Julie A Siegenthaler, Samuel J Pleasure
Sep 29, 2011·Molecular Brain·Hiroyuki KatohHideyuki Okano
Jan 11, 2012·Cell Research·Annita Achilleos, Paul A Trainor
Aug 1, 2012·PloS One·Barbara MurdochMartín I García-Castro
Aug 28, 2012·The Journal of Clinical Investigation·Mayumi NagashimadaHideki Enomoto
Oct 25, 2012·PloS One·Bridget T Jacques-FrickeLaura S Gammill
Nov 17, 2012·PloS One·Erika KagueShannon Fisher
Oct 15, 2013·Scientific Reports·Konstantin L LevitskyJavier Villadiego

❮ Previous
Next ❯

Citations

Oct 24, 2015·Biochemical and Biophysical Research Communications·Takashi SuzukiYoshio Wakamatsu
Feb 12, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Karla TerrazasMichael J Dixon
Aug 8, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Fenglei He, Philippe Soriano
Apr 4, 2017·Genesis : the Journal of Genetics and Development·Guiqian ChenHong-Xiang Liu
Oct 27, 2016·Congenital Anomalies·Mohammed Badrul AminKazushi Aoto
Apr 20, 2018·Genesis : the Journal of Genetics and Development·Julien DebbacheLukas Sommer
Oct 25, 2020·Development·Weiyi Tang, Marianne E Bronner
Oct 7, 2019·Neuro-Chirurgie·M CatalaÉ Arnaud

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.