Megahertz data collection from protein microcrystals at an X-ray free-electron laser

Nature Communications
Marie Luise GrünbeinIlme Schlichting

Abstract

X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates of only 10-120 Hz, placing significant demands on beam time and sample consumption. We describe serial femtosecond crystallography experiments performed at the European XFEL, the first MHz repetition rate XFEL, delivering 1.128 MHz X-ray pulse trains at 10 Hz. Given the short spacing between pulses, damage caused by shock waves launched by one XFEL pulse on sample probed by subsequent pulses is a concern. To investigate this issue, we collected data from lysozyme microcrystals, exposed to a ~15 μm XFEL beam. Under these conditions, data quality is independent of whether the first or subsequent pulses of the train were used for data collection. We also analyzed a mixture of microcrystals of jack bean proteins, from which the structure of native, magnesium-containing concanavalin A was determined.

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Citations

Jan 19, 2019·Journal of Synchrotron Radiation·Aschkan AllahgholiHeinz Graafsma
Mar 2, 2019·Acta Crystallographica. Section D, Structural Biology·Marie Luise Grünbein, Gabriela Nass Kovacs
Mar 12, 2019·Journal of Synchrotron Radiation·J SchulzA P Mancuso
May 11, 2019·Journal of Synchrotron Radiation·Charlotte UetrechtJoachim Schulz
Sep 7, 2019·Journal of Synchrotron Radiation·Steffen HaufSandor Brockhauser
Oct 17, 2019·The FEBS Journal·Feng-Zhu ZhaoDa-Chuan Yin
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Mar 23, 2019·International Journal of Molecular Sciences·Marius Schmidt
Apr 5, 2019·Scientific Data·Marie Luise GrünbeinIlme Schlichting
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Nov 7, 2019·Nature Communications·Chris GisrielNadia A Zatsepin
May 27, 2020·Nature Methods·Marie Luise GrünbeinIlme Schlichting
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Dec 12, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Naghmeh FatemiSimon Kuhn
Nov 23, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Hara JangSeung Jae Lee

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Methods Mentioned

BETA
X-ray

Software Mentioned

COOT
PHASER
PHENIX
CrystFEL
CCP4
AMBIGATOR

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