PMID: 9434722Jan 22, 1998Paper

Megalomicin inhibits HIV-1 replication and interferes with gp160 processing

Virology
E San JoséB Alarcón

Abstract

The inhibitory effects on HIV replication of megalomicin (MGM, an inhibitor of intra-Golgi vesicle transport, have been studied. In experiments at low multiplicity of infection on Jurkat and MT2 cell lines. MGM inhibited the production of p24 antigen, the formation of syncytia, and the induction of apoptosis at concentrations below 5 microM. Furthermore, PCR analysis of genomic DNA showed that, in the presence of MGM, HIV-1 had been eradicated from the culture. MGM also inhibited replication of primary isolates of HIV-1 in blood lymphoblasts and more importantly, at 1 microM, MGM inhibited depletion of CD4+ T cells in cultures of blood lymphocytes from seropositive patients. Finally, MGM inhibited the generation of infectious virions and the processing of the envelope protein precursor gp160 to its mature forms, resulting in the rapid degradation of gp 160. These data suggest that MGM induces a powerful inhibitory effect on HIV-1 replication at nontoxic concentrations by preventing the processing of HIV-1 gp160 envelope protein and the subsequent formation of infectious viral particles.

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Citations

Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Luis R MirandaAlex Franzusoff
Apr 27, 2010·Applied and Environmental Microbiology·Mariana UseglioHugo Gramajo
Dec 5, 2012·Antimicrobial Agents and Chemotherapy·Christopher D GoodmanHugo Gramajo
Jul 19, 2008·Chemistry & Biology·Ali Sadeghi-KhomamiDavid L Jakeman
Jan 31, 2007·Chembiochem : a European Journal of Chemical Biology·Changsheng ZhangJon S Thorson
Nov 2, 2004·The Journal of Biological Chemistry·Alicia MonjasBalbino Alarcón
Jun 26, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Irene ZaldívarEster San José
Oct 3, 1998·Antimicrobial Agents and Chemotherapy·P BonayA Alcina
Nov 7, 2000·Biochimica Et Biophysica Acta·M Moulard, E Decroly

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