Megasphaera elsdenii Lactate Degradation Pattern Shifts in Rumen Acidosis Models

Frontiers in Microbiology
Lianmin ChenHongrong Wang

Abstract

Background:Megasphaera elsdenii is an ecologically important rumen bacterium that metabolizes lactate and relieves rumen acidosis (RA) induced by a high-grain-diet. Understanding the regulatory mechanisms of the lactate metabolism of this species in RA conditions might contribute to developing dietary strategies to alleviate RA. Methods:Megasphaera elsdenii was co-cultured with four lactate producers (Streptococcus bovis, Lactobacilli fermentum, Butyrivibrio fibrisolvens, and Selenomonas ruminantium) and a series of substrate starch doses (1, 3, and 9 g/L) were used to induce one normal and two RA models (subacute rumen acidosis, SARA and acute rumen acidosis, ARA) under batch conditions. The associations between bacterial competition and the shift of organic acids' (OA) accumulation patterns in both statics and dynamics manners were investigated in RA models. Furthermore, we examined the effects of substrate lactate concentration and pH on Megasphaera elsdenii's lactate degradation pattern and genes related to the lactate utilizing pathways in the continuous culture. Results and Conclusion: The positive growth of M. elsdenii and B. fibrisolvens caused OA accumulation in the SARA model to shift from lactate to butyrate and resu...Continue Reading

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Citations

Sep 29, 2020·Frontiers in Microbiology·Steven McLoughlinSinéad M Waters
Oct 11, 2020·Animals : an Open Access Journal From MDPI·M Victoria Sanz-FernandezJavier Martín-Tereso
Jul 28, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Satoshi KoikeYasuo Kobayashi
Jul 6, 2021·Journal of Dairy Science·S KoikeY Kobayashi
Oct 6, 2021·Letters in Applied Microbiology·L C StaicuJ L R Gallego

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
PCA

Software Mentioned

R

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