MeHg Causes Ultrastructural Changes in Mitochondria and Autophagy in the Spinal Cord Cells of Chicken Embryo

Journal of Toxicology
Fabiana F FerreiraYara Maria Rauh Müller

Abstract

Methylmercury (MeHg) is a known neurodevelopmental toxicant, which causes changes in various structures of the central nervous system (CNS). However, ultrastructural studies of its effects on the developing CNS are still scarce. Here, we investigated the effect of MeHg on the ultrastructure of the cells in spinal cord layers. Chicken embryos at E3 were treated in ovo with 0.1 μg MeHg/50 μL saline solution and analyzed at E10. Then, we used transmission electron microscopy (TEM) to identify possible damage caused by MeHg to the structures and organelles of the spinal cord cells. After MeHg treatment, we observed, in the spinal cord mantle layer, a significant number of altered mitochondria with external membrane disruptions, crest disorganization, swelling in the mitochondrial matrix, and vacuole formation between the internal and external mitochondrial membranes. We also observed dilations in the Golgi complex and endoplasmic reticulum cisterns and the appearance of myelin-like cytoplasmic inclusions. We observed no difference in the total mitochondria number between the control and MeHg-treated groups. However, the MeHg-treated embryos showed an increased number of altered mitochondria and a decreased number of mitochondrial f...Continue Reading

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Citations

Jul 21, 2021·Environmental Science & Technology·Claudia CosioAlain Geffard
Apr 28, 2020·Ecotoxicology and Environmental Safety·Na ChenXianjin Xiao
Jan 7, 2022·Chemical Research in Toxicology·Jingjing PanWei Liu

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Methods Mentioned

BETA
light microscopy
GTPases

Software Mentioned

Statistica®
ImageJ

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