Meibomian Glands or Not? Identification of In Vivo and Ex Vivo Confocal Microscopy Features and Histological Correlates in the Eyelid Margin

Journal of Ophthalmology
Yu-Jing Wang, Min Ke

Abstract

In vivo confocal laser scanning microscopy (CLSM) is an emerging diagnostic tool allowing fast and easy microscopic tissue examination. For the diagnostics of pathological eyelid margin lesions, the knowledge of the normal eyelid margin is essential. We examined 18 eyelid margins of healthy humans using the in vivo CLSM device and 10 samples of healthy eyelid margins from donor sites with ex vivo CLSM and compared the findings to the corresponding histological sections of donor sites. Cross-section images of different depths and depths of different skin appendages were measured. The depth observed by in vivo CLSM is less than 150 μm into the eyelid. Images of the epidermis and superficial dermis skin, appendages including hair follicle, and sebaceous catheters can be captured associated with histopathology and ex vivo confocal microscopy. In correlation with histopathology, we identified different layers of the eyelid margin, different layers of the epidermis, and skin appendages by ex vivo confocal microscopy. The study offers an overview of the in vivo confocal microscopy human eyelid margin characteristics in comparison to the standard histological examination and confirms that in vivo CLSM could not observe the meibomian gl...Continue Reading

References

Jun 1, 1995·The Journal of Investigative Dermatology·M RajadhyakshaR R Anderson
Nov 29, 2005·Journal of Clinical Pathology·K O Alsaad, D Ghazarian
Oct 28, 2006·Cornea·Elisabeth M MessmerAnselm Kampik
Feb 27, 2009·Journal of the European Academy of Dermatology and Venereology : JEADV·M ArdigoS González
Apr 23, 2009·The Ocular Surface·Michael A Lemp, Kelly K Nichols
Jun 26, 2009·The British Journal of Ophthalmology·Mario Nubile, Leonardo Mastropasqua
Apr 1, 2011·Investigative Ophthalmology & Visual Science·Alan TomlinsonMurat Dogru
May 17, 2011·Investigative Ophthalmology & Visual Science·Edoardo VillaniRoberto Ratiglia
Feb 14, 2012·Journal of the European Academy of Dermatology and Venereology : JEADV·T MaierC Berking
Jun 22, 2012·The British Journal of Dermatology·E C SattlerC Berking
Aug 11, 2012·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Ozan Yüksel TektaşFriedrich Paulsen
Jun 14, 2013·Investigative Ophthalmology & Visual Science·Edoardo VillaniRoberto Ratiglia
Feb 14, 2014·Experimental Dermatology·Eshter A W WolberinkMarie-Jeanne P Gerritsen
Feb 18, 2014·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Y K BaşaranI S Bağci
Jul 7, 2014·Journal of the American Academy of Dermatology·Elisa CinottiFrédéric Cambazard
Sep 26, 2014·The British Journal of Ophthalmology·Matthieu RandonChristophe Baudouin
May 23, 2015·Journal of Biophotonics·Daniela HartmannTanja von Braunmühl
Mar 22, 2016·Actas dermo-sifiliográficas·M AgozzinoM Ardigò
Apr 6, 2016·BioMed Research International·Vincenzo FasanellaLeonardo Mastropasqua
Oct 28, 2016·Lasers in Surgery and Medicine·Milind RajadhyakshaKishwer S Nehal
Nov 28, 2017·Journal of Cutaneous Pathology·Neda ShahriariAlon Scope
Aug 22, 2018·Investigative Ophthalmology & Visual Science·Scott Zhou, Danielle M Robertson
Mar 7, 2020·The British Journal of Dermatology·J MalvehyS Puig

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Methods Mentioned

BETA
biopsy
confocal microscopy

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