Meiotic Double-Strand Break Proteins Influence Repair Pathway Utilization

Genetics
Nicolas MacaisneJudith L Yanowitz

Abstract

Double-strand breaks (DSBs) are among the most deleterious lesions DNA can endure. Yet, DSBs are programmed at the onset of meiosis, and are required to facilitate appropriate reduction of ploidy in daughter cells. Repair of these breaks is tightly controlled to favor homologous recombination (HR)-the only repair pathway that can form crossovers. However, little is known about how the activities of alternative repair pathways are regulated at these stages. We discovered an unexpected synthetic interaction between the DSB machinery and strand-exchange proteins. Depleting the Caenorhabditis elegans DSB-promoting factors HIM-5 and DSB-2 suppresses the formation of chromosome fusions that arise in the absence of RAD-51 or other strand-exchange mediators. Our investigations reveal that nonhomologous and theta-mediated end joining (c-NHEJ and TMEJ, respectively) and single strand annealing (SSA) function redundantly to repair DSBs when HR is compromised, and that HIM-5 influences the utilization of TMEJ and SSA.

References

May 1, 1974·Genetics·S Brenner
Feb 27, 1999·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T TakanamiA Higashitani
May 8, 2003·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Takako TakanamiAtsushi Higashitani
Aug 28, 2003·Médecine sciences : M/S·Christelle Gally, Jean-Louis Bessereau
Oct 16, 2004·Molecular and Cellular Biology·Jeremy M StarkMaria Jasin
Sep 28, 2007·Nature·Barbara CorneoDavid B Roth
Dec 25, 2007·Cell Research·Meena ShrivastavJac A Nickoloff
Feb 21, 2008·Nature Reviews. Molecular Cell Biology·Dana Branzei, Marco Foiani
Sep 23, 2008·Nature·Eleni P Mimitou, Lorraine S Symington
Sep 24, 2008·Trends in Genetics : TIG·Mitch McVey, Sang Eun Lee
Feb 19, 2010·Nature Reviews. Molecular Cell Biology·Mariko SasakiScott Keeney
Nov 6, 2010·EMBO Reports·Wassim EidStefano Ferrari
Nov 6, 2010·Chromosoma·Bennie B L G Lemmens, Marcel Tijsterman
Feb 18, 2011·Genes & Development·Amitabh V NimonkarStephen C Kowalczykowski
Oct 18, 2011·Nature·Valerie GarciaMatthew J Neale
Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lan N TruongXiaohua Wu
Dec 24, 2013·Nucleic Acids Research·Yi ZhouTanya T Paull

❮ Previous
Next ❯

Citations

Jan 11, 2020·Scientific Reports·Marcello GermoglioAdele Adamo
Dec 3, 2020·Scientific Reports·Pamela SantonicolaAdele Adamo
Jan 7, 2021·Nucleic Acids Research·Talia HatkevichJeff Sekelsky
Dec 17, 2019·Developmental Cell·Varsha BhargavaMichael Buszczak
Mar 20, 2021·Current Biology : CB·David E AlmanzarOfer Rog
Mar 20, 2021·Current Biology : CB·Erik ToraasonDiana E Libuda
Apr 3, 2021·The Journal of Cell Biology·Maria Rosaria Dello StrittoVerena Jantsch
May 27, 2021·Journal of Cell Science·Lexy von Diezmann, Ofer Rog
Aug 22, 2021·Nucleic Acids Research·Ying WangLiangran Zhang
Aug 24, 2021·Frontiers in Cell and Developmental Biology·Marilina RaicesJudith L Yanowitz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.