Meiotic MCM proteins promote and inhibit crossovers during meiotic recombination

BioRxiv : the Preprint Server for Biology
Talia HatkevichKathryn P Kohl

Abstract

Crossover formation as a result of meiotic recombination is vital for proper segregation of homologous chromosomes at the end of meiosis I. In many organisms, crossovers are generated through two crossover pathways: Class I and Class II. To ensure accurate crossover formation, meiosis specific protein complexes regulate the degree in which each pathway is used. One such complex is the mei-MCM complex, which contains MCM (mini chromosome maintenance) and MCM like proteins REC (ortholog of Mcm8), MEI 217, and MEI 218, collectively called the mei MCM complex. The mei-MCM complex genetically promotes Class I crossovers and inhibits Class II crossovers in Drosophila, but it is unclear how individual mei-MCM proteins contribute to crossover regulation. In this study, we perform genetic analyses to understand how specific regions and motifs of mei-MCM proteins contribute to Class I and II crossover formation and distribution. Our analyses show that the long, disordered N terminus of MEI 218 is dispensable for crossover formation, and that mutations that disrupt RECs Walker A and B motifs differentially affect Class I and Class II crossover formation. In Rec Walker A mutants, Class I crossovers exhibit no change, but Class II crossover...Continue Reading

Related Concepts

Angiotensin II
Chromosomes
Drosophila
Meiosis
Culture Media, Conditioned
Class 1
Analysis
Meiotic Recombination
MCM8 protein, human
2-((4-isobutylphenyl)propionyloxy)ethyl methacrylate

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