Meiotic MCM proteins promote and inhibit crossovers during meiotic recombination

BioRxiv : the Preprint Server for Biology
Talia HatkevichKathryn P Kohl


Crossover formation as a result of meiotic recombination is vital for proper segregation of homologous chromosomes at the end of meiosis I. In many organisms, crossovers are generated through two crossover pathways: Class I and Class II. To ensure accurate crossover formation, meiosis specific protein complexes regulate the degree in which each pathway is used. One such complex is the mei-MCM complex, which contains MCM (mini chromosome maintenance) and MCM like proteins REC (ortholog of Mcm8), MEI 217, and MEI 218, collectively called the mei MCM complex. The mei-MCM complex genetically promotes Class I crossovers and inhibits Class II crossovers in Drosophila, but it is unclear how individual mei-MCM proteins contribute to crossover regulation. In this study, we perform genetic analyses to understand how specific regions and motifs of mei-MCM proteins contribute to Class I and II crossover formation and distribution. Our analyses show that the long, disordered N terminus of MEI 218 is dispensable for crossover formation, and that mutations that disrupt RECs Walker A and B motifs differentially affect Class I and Class II crossover formation. In Rec Walker A mutants, Class I crossovers exhibit no change, but Class II crossover...Continue Reading

Related Concepts

Angiotensin II
Culture Media, Conditioned
Class 1
Meiotic Recombination
MCM8 protein, human
2-((4-isobutylphenyl)propionyloxy)ethyl methacrylate

Related Feeds

Aortic Aneurysm

An aortic aneurysm is the weakening and bulging of the blood vessel wall in the aorta. This causes dilatation of the aorta, which is usually asymptomatic but carries the risk of rupture and hemorrhage. Find the latest research on aortic aneurysms here.

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.


Aneurysms are outward distensions or bulges that occurs in a weakened wall of blood vessels. Discover the latest research on aneurysms here.

Related Papers

Current Biology : CB
Danny E Miller, R Scott Hawley
Current Opinion in Genetics & Development
Enrique Martinez-Perez, Monica P Colaiácovo
Biochemical Society Transactions
A Lorenz, M C Whitby
© 2021 Meta ULC. All rights reserved