Meiotic progression of isolated mouse spermatocytes under simulated microgravity

Reproduction : the Official Journal of the Society for the Study of Fertility
Silvia Di AgostinoRaffaele Geremia

Abstract

Progression through the prophase of the first meiotic division can be obtained in culture by treatment of mouse spermatocytes with the serine/threonine phosphatase inhibitor okadaic acid. Chromosome condensation during this G2/M transition involves the activation of the MAPK pathway, which causes the activation of Nek2 and the phosphorylation of the chromatin architectural protein Hmga2. In an effort to set up conditions to allow a spontaneous progression of mouse spermatocytes through meiosis, we have investigated the cell-cycle features of these cells cultured for 24 h with a rotary cell culture system in a humidified atmosphere in a thermostatic incubator to simulate a microgravity environment. Morphological analysis of nuclear squashes indicated a 2-fold increase in late-pachytene spermatocytes with highly condensed chromosomes, and a contemporaneous decrease of mid-pachytene cells with less condensed chromatin. Microgravity induced a 2-fold activation of the cyclinB-cdc2 complex, confirming at the molecular level that cell-cycle progression had occurred. Moreover, using immuno-kinase assays with specific substrates we have demonstrated that the meiotic progression obtained under microgravity conditions is accompanied by ac...Continue Reading

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Citations

Jan 8, 2008·Molecular and Cellular Endocrinology·Tal Almog, Zvi Naor
Jul 19, 2015·General and Comparative Endocrinology·B R Lackey, S L Gray
Nov 22, 2008·The Journal of Pathology·Federica BarbagalloClaudio Sette
Aug 21, 2014·BioMed Research International·Francesca FerrantiGiulia Ricci
Mar 19, 2014·Biochimica Et Biophysica Acta·Mazen ShihanGeorgios Scheiner-Bobis
Jul 2, 2005·Journal of Cellular Physiology·Elena ViciniClaudio Sette
Jun 16, 2017·Scientific Reports·Elisa CirelliPaola Grimaldi

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