Meiotic state of bovine oocytes is regulated by interactions between cAMP, cumulus, and granulosa

Molecular Reproduction and Development
Huseyin AktasNeal L First

Abstract

Bovine oocytes are arrested at the prophase of first meiotic cell cycle. Meiosis resumes in oocytes of pre-ovulatory follicles upon LH surge. However, oocytes from secondary follicles spontaneously resume meiosis in the absence of hormones if removed from the follicle and cultured in vitro. The nature of meiotic arrestor in bovine follicles is poorly understood. In this study we investigated the role of cell-cell interactions between granulosa and cumulus cells and the oocyte in mediating maintenance of meiotic arrest by cAMP. We sorted oocytes as granulosa-cumulus oocyte complexes (GCOC) if surrounded with cumulus cells attached to a large granulosa investment or cumulus oocytes complexes (COC) if surrounded with cumulus cells only and investigated the role cAMP in maintenance of meiotic arrest in these oocytes under various conditions. In hormone- and serum-free medium both GCOC and COC enclosed oocytes resumed meiosis. When [cAMP](i) was elevated with addition of invasive adenylate cyclase (iAC) GCOC enclosed oocytes were maintained in the prophase with intact germinal vesicle (GV) while COC enclosed oocytes underwent GV breakdown (GVBD). iAC elevated [cAMP](i) in both types of oocytes to the same level. If oocytes were libe...Continue Reading

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Citations

Dec 23, 2011·ISRN Obstetrics and Gynecology·Jacira Ribeiro Campos, Ana Carolina Japur de Sá Rosa-E-Silva
Sep 13, 2014·Theriogenology·Irina Y LebedevaMette Schmidt
Jul 2, 2005·Animal Reproduction Science·F SchneiderW Kanitz
Jul 16, 2004·The Journal of Clinical Investigation·Silvia MasciarelliVincent Manganiello
Mar 6, 2009·Reproduction, Fertility, and Development·Dawit TesfayePatrick Lonergan

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