Abstract
To improve the survival and/or differentiation of grafted BMSCs (bone marrow stem cells) represents one of the challenges for the promising cell-based therapy. Considerable reports have implicated Sal B (salvianolic acid B), a potent aqueous extract of Salvia miltiorrhiza, in enhancing the survival of cells under various conditions. In this study, we investigated the effect of Sal B on H₂O₂-induced apoptosis in rat BMSCs, focusing on the survival signalling pathways. Results indicated that the MEK [MAPK (mitogen-activated protein kinase)/ERK (extracellular-signal-regulated kinase) kinase] inhibitor (PD98059) and 10 μM Sal B remarkably prevented BMSCs from H₂O₂-induced apoptosis through attenuating caspase-3 activation, which is accompanied by the significant up-regulation of Bcl-2. In addition, the ROS (reactive oxygen species) accumulation was also reduced after Sal B treatment. Furthermore, Sal B inhibited the ERK1/2 phosphorylations stimulated by H₂O₂. Taken together, our results showed that H₂O₂-induced apoptosis in BMSCs via the ROS/MEK/ERK1/2 pathway and Sal B may exert its cytoprotection through mediating the pathway.
References
Jan 1, 1996·Brain Research Bulletin·E M Grasbon-FrodlP Brundin
Jan 14, 1999·Journal of Neurochemistry·N R Bhat, P Zhang
Jul 6, 2000·Experimental Neurology·O HanssonP Brundin
Sep 20, 2000·The Journal of Biological Chemistry·X WangN J Holbrook
Jan 5, 2002·Circulation·Catalin TomaPaul D Kessler
Feb 20, 2002·Journal of Molecular and Cellular Cardiology·Jochen Müller-EhmsenLarry Kedes
Jul 29, 2004·DNA Repair·Ari Barzilai, Ken-Ichi Yamamoto
Jul 31, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Noritoshi NagayaSoichiro Kitamura
Jul 14, 2005·Journal of Applied Toxicology : JAT·Yong Keun KimJong Min Kim
Oct 29, 2005·Stem Cells·Weiquan ZhuXi Chen
Jan 21, 2006·Stem Cells·Regina EbertFranz Jakob
Jan 28, 2006·Journal of Cellular Biochemistry·Yuh-Lien ChenShing-Jong Lin
Jul 4, 2006·Free Radical Biology & Medicine·Alexandra Stolzing, Andrew Scutt
Oct 20, 2006·Journal of Neurosurgery. Spine·David S DitorLynne C Weaver
Dec 19, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Chang-Suo LiuJun-Tian Zhang
Jun 2, 2007·Stem Cells·Shih-Chieh HungDarwin J Prockop
Jun 15, 2007·Neurochemical Research·Venkata Ramesh DasariDzung H Dinh
Sep 22, 2007·The International Journal of Biochemistry & Cell Biology·Lin-Lin TianSheng-Qi Wang
Sep 29, 2007·Stem Cells·Donald G Phinney, Darwin J Prockop
Nov 15, 2007·Cell Cycle·Donald G Phinney
Dec 20, 2007·PloS One·Chen-Li LiuJian-Dong Huang
Jan 29, 2008·Current Stem Cell Research & Therapy·Steven A HardyStefan A Przyborski
May 10, 2008·Stem Cells·Céline MiasDaniel Cussac
Sep 26, 2008·Cardiovascular Research·Hong-Li WuXue-Jun Li
Mar 20, 2009·Journal of Chromatography. a·Shidong KanDaijie Chen
Jul 25, 2009·Cellular and Molecular Life Sciences : CMLS·Frank Weinberg, Navdeep S Chandel
Oct 3, 2009·The FEBS Journal·Mikhail F Alexeyev
Citations
Sep 4, 2012·Acta Pharmacologica Sinica·Wen-yu Wu, Yi-ping Wang
Apr 12, 2012·PloS One·Liao CuiTie Wu
Jun 17, 2014·Neurochemistry International·Huimin YuZhicheng Liu
Apr 22, 2015·Molecular Medicine Reports·Peng GaoQingping Wen
Jan 7, 2011·Journal of Oncology·Yuan ZhaoXinbin Gu
Jun 5, 2012·The Journal of Surgical Research·Guo-zheng PanGui-hua Chen
Oct 23, 2012·European Journal of Pharmacology·Nan ZhangJingxian Yang
Mar 25, 2014·The International Journal of Biochemistry & Cell Biology·Daohua XuGang Li
Apr 18, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jian-Ping DaiYun Su
Apr 27, 2019·Connective Tissue Research·Hui-Shen YanPing Bo
Jun 5, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Dongtao LiXiaoming Wang
Dec 4, 2019·FEBS Open Bio·Liang MaCao Yang
Feb 11, 2020·Frontiers in Pharmacology·Yong-Zhi GuoRong-Rong He
May 6, 2020·Drug Design, Development and Therapy·Qingbin WuRuijuan Xiu
Jul 22, 2020·Cytotechnology·Jiaxing LiuWen-Song Tan
Dec 22, 2020·Frontiers in Pharmacology·Zhun XiaoPing Liu