MEK/ERK pathway mediates cytoprotection of salvianolic acid B against oxidative stress-induced apoptosis in rat bone marrow stem cells

Cell Biology International
Biyan LuJianqiang Feng

Abstract

To improve the survival and/or differentiation of grafted BMSCs (bone marrow stem cells) represents one of the challenges for the promising cell-based therapy. Considerable reports have implicated Sal B (salvianolic acid B), a potent aqueous extract of Salvia miltiorrhiza, in enhancing the survival of cells under various conditions. In this study, we investigated the effect of Sal B on H₂O₂-induced apoptosis in rat BMSCs, focusing on the survival signalling pathways. Results indicated that the MEK [MAPK (mitogen-activated protein kinase)/ERK (extracellular-signal-regulated kinase) kinase] inhibitor (PD98059) and 10 μM Sal B remarkably prevented BMSCs from H₂O₂-induced apoptosis through attenuating caspase-3 activation, which is accompanied by the significant up-regulation of Bcl-2. In addition, the ROS (reactive oxygen species) accumulation was also reduced after Sal B treatment. Furthermore, Sal B inhibited the ERK1/2 phosphorylations stimulated by H₂O₂. Taken together, our results showed that H₂O₂-induced apoptosis in BMSCs via the ROS/MEK/ERK1/2 pathway and Sal B may exert its cytoprotection through mediating the pathway.

References

Sep 20, 2000·The Journal of Biological Chemistry·X WangN J Holbrook
Feb 20, 2002·Journal of Molecular and Cellular Cardiology·Jochen Müller-EhmsenLarry Kedes
Jul 29, 2004·DNA Repair·Ari Barzilai, Ken-Ichi Yamamoto
Jul 31, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Noritoshi NagayaSoichiro Kitamura
Jul 14, 2005·Journal of Applied Toxicology : JAT·Yong Keun KimJong Min Kim
Jul 4, 2006·Free Radical Biology & Medicine·Alexandra Stolzing, Andrew Scutt
Dec 19, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Chang-Suo LiuJun-Tian Zhang
Sep 22, 2007·The International Journal of Biochemistry & Cell Biology·Lin-Lin TianSheng-Qi Wang
Jan 29, 2008·Current Stem Cell Research & Therapy·Steven A HardyStefan A Przyborski
Jul 25, 2009·Cellular and Molecular Life Sciences : CMLS·Frank Weinberg, Navdeep S Chandel

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Citations

Mar 25, 2014·The International Journal of Biochemistry & Cell Biology·Daohua XuGang Li
Apr 18, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jian-Ping DaiYun Su
Dec 22, 2020·Frontiers in Pharmacology·Zhun XiaoPing Liu

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