Mel-18 interacts with RanGAP1 and inhibits its sumoylation.

Biochemical and Biophysical Research Communications
Jie Zhang, Kevin D Sarge

Abstract

Our previous results showed that the polycomb protein mel-18 binds to a protein called HSF2 and inhibits HSF2 sumoylation, thereby functioning as an anti-SUMO E3 factor. This study also suggested that mel-18 regulates the sumoylation of other cellular proteins, but the identities of these other proteins were unknown. Here we show that mel-18 interacts with the RanGAP1 protein and inhibits its sumoylation, and that these activities do not require the RING domain of mel-18. The results also show that RanGAP1 sumoylation is decreased during mitosis, and that this is associated with increased interaction between RanGAP1 and mel-18 during this stage of the cell cycle. Intriguingly, this regulatory relationship is the opposite of that found for mel-18 and HSF2, in which the interaction between these two proteins decreases during mitosis, resulting in elevated HSF2 sumoylation. The results of this study strengthen the conclusion that mel-18 functions as an anti-SUMO E3 factor, and extend its targets to include regulation of the sumoylation of the important cellular protein RanGAP1.

References

Jan 1, 1993·DNA Sequence : the Journal of DNA Sequencing and Mapping·H AsanoM Kanno
Oct 27, 1997·The Journal of Biological Chemistry·E S Johnson, G Blobel
Dec 31, 1997·FEBS Letters·J M DesterroR T Hay
Jan 2, 2001·The Journal of Biological Chemistry·M S RodriguezR T Hay
Oct 5, 2001·Molecular Cell·T KahyoH Yasuda
Jan 17, 2002·Cell·Andrea PichlerFrauke Melchior
Feb 21, 2002·The Journal of Cell Biology·Jomon JosephMary Dasso
Apr 8, 2003·Cell·Michael H KageyDavid Wotton
Apr 6, 2005·Molecular Cell·Ronald T Hay
Aug 13, 2005·Current Opinion in Genetics & Development·Grace Gill
Jun 7, 2006·Annual Review of Cell and Developmental Biology·Oliver KerscherMark Hochstrasser
May 15, 2007·Trends in Biochemical Sciences·Debaditya Mukhopadhyay, Mary Dasso
Nov 15, 2007·Nature Reviews. Molecular Cell Biology·Ruth Geiss-Friedlander, Frauke Melchior
Jan 24, 2008·The Journal of Biological Chemistry·Jie ZhangKevin D Sarge

❮ Previous
Next ❯

Citations

Sep 3, 2009·Epigenetics & Chromatin·Hanneke E C NiessenJan Willem Voncken
Sep 2, 2010·Cell Stem Cell·Martin Sauvageau, Guy Sauvageau
Mar 31, 2015·The Journal of Clinical Investigation·Jeong-Yeon LeeGu Kong

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.