Mel 1a melatonin receptor expression is regulated by protein kinase C and an additional pathway addressed by the protein kinase C inhibitor Ro 31-8220 in ovine pars tuberalis cells

Endocrinology
P BarrettP J Morgan

Abstract

The expression of the melatonin receptor is positively regulated by cAMP and negatively regulated by melatonin in the ovine pars tuberalis (PT). Furthermore, when PT cells are dispersed in primary culture, both messenger RNA (mRNA) and protein levels spontaneously increase through a process that can be blocked by melatonin, but does not involve cAMP. This suggests that other second messengers may be regulated by melatonin, which, in turn, regulates melatonin receptor mRNA and protein levels. In this study using ribonuclease protection assays, ligand binding, protein kinase C (PKC), and cAMP analysis, we demonstrate that the levels of Mel 1a mRNA and protein expression in ovine PT are reduced by phorbol 12-myristate 13-acetate in a cAMP-independent process. This is indicative of an inhibitory role for PKC in receptor regulation. Melatonin, however, does not act through PKC activation to reduce Mel 1a mRNA or protein levels. Basal PKC activity in PT cells can be inhibited by the PKC inhibitor Ro 31-8220, and this suggests that basal PKC activity may suppress Mel 1a receptor expression. Paradoxically, however, Ro 31-8220 also inhibits melatonin receptor mRNA and protein levels in PT cells by a cAMP-independent mechanism. This sugg...Continue Reading

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Citations

Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Antonio Carrillo-VicoJuan M Guerrero
Mar 28, 2001·Annals of the New York Academy of Sciences·C Carlberg
Mar 28, 2001·Annals of the New York Academy of Sciences·J M GuerreroJ R Calvo
Mar 31, 2015·PloS One·Fanny SöderquistJanet L Cunningham
Jan 29, 2021·Frontiers in Endocrinology·Elia CianiRomain Fontaine

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