Melamine-Induced Decomposition and Anti-FRET Effect from a Self-Assembled Complex of Rhodamine 6G and DNA-Stabilized Silver Nanoclusters Used for Dual-Emitting Ratiometric and Naked-Eye-Visible Fluorescence Detection

Journal of Agricultural and Food Chemistry
Yongxin FuRijun Gui

Abstract

In this work, blue-emitting silver nanoclusters (AgNCs) were prepared in a matrix of single-stranded deoxyribonucleic acid (DNA) on the basis of ambient hydrothermal reactions. DNA acted as the stabilizer or coating agent, and NaBH4 was used as the reducing agent. Through the interactions between rhodamine 6G (Rh6G) and the synthesized DNA-AgNCs, the self-assembled complex of DNA-AgNC-Rh6G was generated. Meanwhile, fluorescence emission of AgNCs was weakened as a result of fluorescence-resonance-energy transfer (FRET) from AgNCs (donor) to Rh6G (acceptor). In the DNA-AgNC-Rh6G complex aqueous suspension, the addition of melamine induced obvious emission recovery of AgNCs and fluorescence decrease of Rh6G, attributable to melamine-induced decomposition of the self-assembled complex and anti-FRET effects. There was a well-plotted linear relationship of ratiometric fluorescence intensities ( IAgNCs/ IRh6G) versus melamine concentration in the range of 0.1-10 μM, with a low detection limit of 25 nM. Responses of IAgNCs/ IRh6G to melamine were highly selective and sensitive over potential interferents. A novel dual-emitting ratiometric fluorescence sensor of melamine was facilely constructed on the basis of the DNA-AgNC-Rh6G complex...Continue Reading

References

Mar 19, 2008·Journal of the American Chemical Society·Chris I RichardsRobert M Dickson
Oct 3, 2008·Chemical Communications : Chem Comm·Akira OnoYoshiyuki Tanaka
Apr 27, 2010·Analytica Chimica Acta·Gopalakrishnan Venkatasami, John R Sowa
May 8, 2010·Chemical Communications : Chem Comm·Guo-Yu LanHuan-Tsung Chang
Aug 12, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·H MiaoY N Wu
Feb 12, 2011·Chemical Communications : Chem Comm·Zhengzhen HuangXiaogang Qu
May 12, 2012·Biosensors & Bioelectronics·Shuang HanGuobao Xu
Jun 13, 2012·Toxicology Letters·Jian-dong LiuZhi-xiong Zhuang
Jan 28, 2014·ACS Applied Materials & Interfaces·Lingling LiJun-Jie Zhu
Apr 22, 2015·Physical Chemistry Chemical Physics : PCCP·Youngil ParkHsing-Lin Wang
Nov 15, 2017·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Nan GaoFangying Wu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.