Melanin-concentrating hormone binding sites in human SVK14 keratinocytes

Biochemical and Biophysical Research Communications
J L BurgaudJ L Nahon

Abstract

Melanin concentrating hormone (MCH) is a cyclic peptide which regulates a broad array of functions in the mammalian brain and it may act as a paracrine factor in peripheral organs. In these studies a radiolabeled MCH derivative, the [125I]-[Phe13, Tyr19]-MCH, was synthesized and used as a tracer to perform binding experiments. A number of human or rodent cell lines displayed specific binding with [125I]-[Phe13, Tyr19]-MCH, the highest binding capacity being observed with human SVK14 keratinocytes. Saturation binding analysis with SVK14 cells indicated about 10,000 MCH binding sites per cell and a Kd of 0.7 nM for [125I]-[Phe13, Tyr19]-MCH. Surprisingly, the iodinated [Phe13, Tyr19]-MCH displayed about 10-fold higher affinity (Ki approximately 3.0 nM) for the putative MCH receptor than the noniodinated form (Ki approximately 25-30 nM). Competition binding analyses comparing various MCH-related peptides revealed a similar low binding potency for all these peptides (Ki approximately 65-160 nM). Strikingly, rat ANP and rat/human CNP but not rat BNP displaced [125I]-[Phe13, Tyr15]-MCH with Ki approximately 210-365 nM and may be due to topological similarities instead of partial sequence identities between MCH and some of the natriur...Continue Reading

References

May 8, 1992·The Journal of Comparative Neurology·J C BittencourtP E Sawchenko
Apr 1, 1990·Molecular Endocrinology·F PresseW Vale
Feb 27, 1984·FEBS Letters·G ThibaultM Chrétien
Jun 1, 1993·Brain Research. Molecular Brain Research·C BretonJ L Nahon
Jan 1, 1995·Journal of Peptide Science : an Official Publication of the European Peptide Society·R Drozdz, A N Eberle
Jul 18, 1997·Biochemical and Biophysical Research Communications·P A Coulombe
Apr 1, 1994·Trends in Endocrinology and Metabolism : TEM·B I Baker
Jun 1, 1993·Molecular and Cellular Neurosciences·C BretonJ L Nahon

❮ Previous
Next ❯

Citations

May 26, 2001·British Journal of Pharmacology·V AudinotJ A Boutin
Apr 3, 2002·The Journal of Clinical Investigation·E Helen KempPhilip F Watson
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·S AnK Dai
Feb 12, 2004·The Journal of Investigative Dermatology. Symposium Proceedings·I Caroline Le PoolePranab K Das
Sep 22, 2006·Molecular and Cellular Endocrinology·David A SchwarzRichard A Maki
Feb 13, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Jun 12, 2002·Canadian Journal of Physiology and Pharmacology·Jean A BoutinJean-Luc Fauchère
May 19, 2000·Annals of the New York Academy of Sciences·T SuplyJ L Nahon
Dec 31, 2002·Journal of Receptor and Signal Transduction Research·Sophie E SchlumbergerAlex N Eberle
Mar 29, 2001·The Journal of Biological Chemistry·J HillA I Muir
Nov 19, 2010·Journal of Receptor and Signal Transduction Research·Alex N EberleUrs Zumsteg
Apr 12, 2003·Journal of Receptor and Signal Transduction Research·Sophie E SchlumbergerAlex N Eberle
Mar 11, 1999·Journal of Receptor and Signal Transduction Research·E HintermannA N Eberle

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.