Abstract
Melanin concentrating hormone (MCH) is a cyclic peptide which regulates a broad array of functions in the mammalian brain and it may act as a paracrine factor in peripheral organs. In these studies a radiolabeled MCH derivative, the [125I]-[Phe13, Tyr19]-MCH, was synthesized and used as a tracer to perform binding experiments. A number of human or rodent cell lines displayed specific binding with [125I]-[Phe13, Tyr19]-MCH, the highest binding capacity being observed with human SVK14 keratinocytes. Saturation binding analysis with SVK14 cells indicated about 10,000 MCH binding sites per cell and a Kd of 0.7 nM for [125I]-[Phe13, Tyr19]-MCH. Surprisingly, the iodinated [Phe13, Tyr19]-MCH displayed about 10-fold higher affinity (Ki approximately 3.0 nM) for the putative MCH receptor than the noniodinated form (Ki approximately 25-30 nM). Competition binding analyses comparing various MCH-related peptides revealed a similar low binding potency for all these peptides (Ki approximately 65-160 nM). Strikingly, rat ANP and rat/human CNP but not rat BNP displaced [125I]-[Phe13, Tyr15]-MCH with Ki approximately 210-365 nM and may be due to topological similarities instead of partial sequence identities between MCH and some of the natriur...Continue Reading
References
Jan 1, 1992·Life Sciences·T O MatsunagaM E Hadley
Aug 15, 1992·The Biochemical Journal·F CheclerJ P Vincent
May 8, 1992·The Journal of Comparative Neurology·J C BittencourtP E Sawchenko
Apr 1, 1990·Molecular Endocrinology·F PresseW Vale
Oct 1, 1989·Endocrinology·J L NahonW Vale
Jul 1, 1989·Peptides·T O MatsunagaM E Hadley
Sep 1, 1989·Endocrinology·J M VaughanW Vale
Oct 1, 1987·Journal of Cellular Physiology·P D PhillipsV J Cristofalo
May 31, 1988·Biochemistry·J A FehrentzC Carelli
Nov 1, 1985·Peptides·B I BakerJ Girard
Feb 27, 1984·FEBS Letters·G ThibaultM Chrétien
Sep 22, 1983·Nature·H KawauchiB I Baker
Apr 1, 1995·Neuroendocrinology·G Hervieu, J L Nahon
Feb 13, 1995·FEBS Letters·R DrozdzA N Eberle
Jun 1, 1993·Brain Research. Molecular Brain Research·C BretonJ L Nahon
Feb 1, 1996·Endocrinology·G HervieuJ L Nahon
Mar 21, 1996·Nature·D QuE Maratos-Flier
Jul 1, 1996·Endocrinology·C RovereP Kitabgi
Oct 1, 1996·Endocrinology·G ToumaniantzJ L Nahon
Apr 1, 1996·Neuroscience·F PresseJ L Nahon
Jan 1, 1995·Journal of Receptor and Signal Transduction Research·R Drozdz, A N Eberle
Jan 1, 1997·Endocrinology·M RossiS R Bloom
Jun 1, 1997·Brain Research. Molecular Brain Research·A VialeJ L Nahon
Jan 1, 1995·Journal of Peptide Science : an Official Publication of the European Peptide Society·R Drozdz, A N Eberle
Jul 18, 1997·Biochemical and Biophysical Research Communications·P A Coulombe
Apr 1, 1994·Trends in Endocrinology and Metabolism : TEM·B I Baker
Jun 1, 1993·Molecular and Cellular Neurosciences·C BretonJ L Nahon
Citations
May 26, 2001·British Journal of Pharmacology·V AudinotJ A Boutin
Apr 3, 2002·The Journal of Clinical Investigation·E Helen KempPhilip F Watson
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·S AnK Dai
Feb 12, 2004·The Journal of Investigative Dermatology. Symposium Proceedings·I Caroline Le PoolePranab K Das
May 16, 2009·Peptides·E Helen Kemp, Anthony P Weetman
Oct 13, 2004·Peptides·Yuguang Shi
Oct 13, 2004·Peptides·Alex N EberleUrs Zumsteg
Oct 13, 2004·Peptides·Bruno CardinaudJean-Louis Nahon
Sep 22, 2006·Molecular and Cellular Endocrinology·David A SchwarzRichard A Maki
Jul 25, 2006·Comptes rendus biologies·Jean-Louis Nahon
Nov 11, 1999·Brain Research·M RossiS R Bloom
Oct 7, 2000·Neuropeptides·E KokkotouE Maratos-Flier
Mar 30, 2001·The Journal of Biological Chemistry·V AudinotJ A Boutin
Jul 27, 1999·Nature·J ChambersH M Sarau
Nov 6, 2001·Journal of Receptor and Signal Transduction Research·E HintermannA N Eberle
Feb 13, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Jun 12, 2002·Canadian Journal of Physiology and Pharmacology·Jean A BoutinJean-Luc Fauchère
Mar 7, 2001·The Journal of Clinical Endocrinology and Metabolism·K TakahashiS Shibahara
May 19, 2000·Annals of the New York Academy of Sciences·T SuplyJ L Nahon
Dec 31, 2002·Journal of Receptor and Signal Transduction Research·Sophie E SchlumbergerAlex N Eberle
Mar 29, 2001·The Journal of Biological Chemistry·J HillA I Muir
Nov 19, 2010·Journal of Receptor and Signal Transduction Research·Alex N EberleUrs Zumsteg
Feb 13, 2002·The Journal of Biological Chemistry·Maria A BednarekScott D Feighner
Apr 12, 2003·Journal of Receptor and Signal Transduction Research·Sophie E SchlumbergerAlex N Eberle
Mar 11, 1999·Journal of Receptor and Signal Transduction Research·E HintermannA N Eberle
Aug 2, 2001·Biochemistry·M A BednarekL H Van der Ploeg
May 16, 2002·Biochemistry·Maria A BednarekScott D Feighner