PMID: 9440313Jan 24, 1998Paper

Melanin-porphyrin interaction monitored by delayed luminescence and photoacoustics

Journal of Photochemistry and Photobiology. B, Biology
D WróbelTadeusz Sarna

Abstract

Even though melanin is commonly viewed as a photoprotective agent, the molecular mechanism of the melanin-related photoprotective action remains unclear. We studied the interaction of a synthetic dopa melanin with positively and negatively charged porphyrins using an array of spectroscopic techniques. Thus absorption, fluorescence, time-resolved delayed luminescence in the microsecond time range, and photoacoustic spectra at different modulation frequencies of both porphyrins and their mixtures with dopa melanin were measured in buffered aqueous solution at room temperature. It has been confirmed that dopa melanin forms a complex with the cationic porphyrin in its ground state, which significantly modifies the optical properties of the dye molecule. Although no such complex can be detected for the anionic porphyrin, some interaction between melanin and the porphyrin molecule can be detected in its excited state. Both porphyrins show delayed luminescence with a decay time of 35-38 microseconds, which is substantially reduced by melanin. Our photoacoustic measurements indicate that upon photoexcitation, melanin efficiently liberates heat. Our data also suggest that the thermal properties of melanin can be modified by porphyrins, ...Continue Reading

References

Apr 1, 1992·Photochemistry and Photobiology·M OuzafeJ Chambron
Mar 1, 1990·Journal of Biochemical and Biophysical Methods·A AndreoniP Russo
Aug 6, 1986·Biochimica Et Biophysica Acta·T SarnaT G Truscott
Oct 1, 1988·Medicinal Research Reviews·G Prota
Apr 1, 1994·Photochemistry and Photobiology·I A VitkinR R Anderson
Nov 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·A LosiC Viappiani

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Citations

Dec 13, 2005·Free Radical Biology & Medicine·Mariusz ZarebaJanice M Burke

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