Melanocytes from dark and light skin respond differently after ultraviolet B irradiation: effect of keratinocyte-conditioned medium

Photodermatology, Photoimmunology & Photomedicine
Saioa LópezIsabel Smith-Zubiaga

Abstract

The response to the damage provoked by exposure to UV radiation is mediated by melanocytes and a network of paracrine factors produced by keratinocytes, and it varies among individuals of different geographical origin and skin colour. The mechanisms underlying this differential response, however, have not been completely elucidated. We characterized the differential behaviour of melanocytes (proliferation and differentiation/melanogenesis) from both dark- and light-skinned individuals in response to ultraviolet B (UVB) irradiation, cultured with and without keratinocyte-conditioned medium (KCM). ELISA assays and real-time quantitative PCR were used to assess the production of keratinocyte-derived factors. After UVB irradiation, dark melanocytes showed a decreased proliferation consistent with the highly differentiated state inferred by the increased dendricity of the cells and higher levels of melanogenic genes expression, whereas light melanocytes showed an increase in proliferation in accord with a less differentiated state and decreased melanogenesis levels. KCM induced melanogenesis in dark melanocytes after UVB irradiation, but not in light-pigmented melanocytes. Proliferation and differentiation are coordinated in respons...Continue Reading

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Citations

Sep 29, 2019·Pigment Cell & Melanoma Research·Christopher R ArnetteKathleen J Green
Feb 7, 2020·Photodermatology, Photoimmunology & Photomedicine·Jin Cheol KimHee Young Kang
Jul 18, 2017·Journal of Tissue Engineering and Regenerative Medicine·Teresa MichalczykErnst Reichmann

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