PMID: 19132244Jan 10, 2009Paper

Melanoma cell adhesion can be blocked by heparin in vitro: suggestion of VLA-4 as a novel target for antimetastatic approaches

Thrombosis and Haemostasis
Juliane FritzscheGerd Bendas

Abstract

The clinical benefit of heparin in cancer patients to prolong survival can be attributed to non-anticoagulant mechanisms. Since adhesion molecules are crucially involved in tumour cell metastasis, their inhibition offers an attractive approach for interfering with the metastatic cascade. Heparin is known to attenuate metastasis in a selectin-dependent manner and possesses a variety of additional effects that are thought to influence tumour cell dissemination, proliferation, and angiogenesis. We investigated the adhesion behaviour of B16F10 melanoma cells in vitro regarding selectin- and VLA-4/VCAM-1-mediated binding to get an insight into underlying mechanisms of melanoma cell metastasis. We show that B16F10 cells display binding ability to P- and L-selectin as well as to isolated platelets. In contrast, B16F10 cells did not adhere to immobilized P-selectin under flow. This contributes to recent findings that elucidate a major role of platelet P-selectin for microemboli formation and thus, facilitating metastasis. In contrast, B16F10 cells adhered to endothelial cells under flow, which could partly be inhibited by a function-blocking anti-VCAM-1 mAb. To emphasize VCAM-1 function, we analyzed cell adhesion at immobilized VCAM-1 ...Continue Reading

Citations

Apr 17, 2012·International Journal of Cell Biology·Gerd Bendas, Lubor Borsig
Feb 21, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Tarek Sousou, Alok A Khorana
Jul 16, 2014·Seminars in Oncology·Lucy A Coupland, Christopher R Parish
Nov 7, 2013·Journal of Cell Communication and Signaling·James Hutchenreuther
Aug 4, 2015·International Journal of Cancer. Journal International Du Cancer·Diego CorreaArnold I Caplan
Sep 1, 2015·Pharmacological Reports : PR·Agnieszka BlazejczykJoanna Wietrzyk
Mar 19, 2014·Thrombosis Research·Martin SchlesingerGerd Bendas
May 2, 2013·Journal of Drug Delivery·Joana R ViolaManfred Ogris
Jun 9, 2017·British Journal of Clinical Pharmacology·Jenneke LeentjensCornelis Kramers
May 16, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ana B García-MartínRuth Lyck
Feb 3, 2016·Molecular Medicine·Jagoda K Wrobel, Michal Toborek

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