Melanoma cells become resistant to NK-cell-mediated killing when exposed to NK-cell numbers compatible with NK-cell infiltration in the tumor

European Journal of Immunology
Mirna BalsamoMassimo Vitale

Abstract

During the past few years, a number of studies reported that different melanoma cell lines could be extensively lysed in vitro by IL-2-activated NK cells at appropriate effector/target ratios. Here, we show, by histological evaluation of different melanoma lesions, that NK/target-cell ratios compatible with those allowing efficient melanoma cell killing in vitro are hardly reached at the tumor site. We then investigated the outcome of cocultures established at low NK/melanoma cell ratios. After initial NK-mediated lysis, residual melanoma cells acquired resistance to IL-2-activated NK cells. This reflected primarily an increased expression, on melanoma cells, of classical and nonclassical HLA class I molecules, accompanied by a partial downregulation of NKG2D-ligands, and was dependent on NK-mediated IFN-γ release. Consistently, melanoma lesions showed a higher HLA class I expression on tumor cells that were proximal to infiltrating NK cells. In long-term cocultures, the "protective phenotype" acquired by melanoma cells was lost over time. However, this phenomenon was counteracted by downregulation of relevant activating receptors in cocultured NK cells. Analysis of different NK-cell-activating cytokines indicated that IL-15 ca...Continue Reading

References

Feb 24, 2000·The Journal of Experimental Medicine·M J SmythD I Godfrey
Mar 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·S KimW M Yokoyama
Apr 15, 2000·Seminars in Immunology·M López-BotetT Bellón
Feb 4, 2003·Nature Reviews. Immunology·Giorgio Trinchieri
Mar 6, 2003·Immunogenetics·Rosario MarínFederico Garrido
Mar 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Roberta CastriconiAlessandro Moretta
Aug 13, 2003·The Journal of Experimental Medicine·Cristina BottinoAlessandro Moretta
Jun 29, 2004·Molecular Immunology·Cristina BottinoAlessandro Moretta
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Roberta CastriconiCristina Bottino
Nov 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Guido FerlazzoChristian Münz
Mar 18, 2005·Annual Review of Immunology·Lewis L Lanier
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emanuela MarcenaroAlessandro Moretta
Oct 4, 2005·Trends in Immunology·Alessandro MorettaLorenzo Moretta
Oct 6, 2005·The Journal of Experimental Medicine·Robbie B MailliardPawel Kalinski
Jun 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lei XuRichard O Hynes
Aug 15, 2006·Seminars in Cancer Biology·Anja Fuchs, Marco Colonna
Aug 11, 2007·Seminars in Cancer Biology·Vera RebmannHans Grosse-Wilde
Mar 13, 2008·Blood·Edgardo D CarosellaJoel Lemaoult
Apr 22, 2008·Nature Immunology·Eric VivierSophie Ugolini
Jul 25, 2008·Blood·Michael A Caligiuri
Dec 18, 2008·International Journal of Cancer. Journal International Du Cancer·Nicole SchwinnAnnette Paschen
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gili G HalfteckOfer Mandelboim
Apr 8, 2009·The Journal of Clinical Investigation·Tadepally LakshmikanthFrancesco Colucci
May 12, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Willem W Overwijk, Kimberly S Schluns
Jun 6, 2009·International Immunology·Gabriella PietraMaria Cristina Mingari
Jun 17, 2009·The Journal of Experimental Medicine·Cameron S BrandtSteven D Levin
Nov 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·Mirna BalsamoMassimo Vitale
Nov 9, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Esther M de KruijfPeter J K Kuppen
Dec 2, 2010·Proceedings of the National Academy of Sciences of the United States of America·Francesca BelloraCristina Bottino
Jan 8, 2011·Science·Eric VivierSophie Ugolini

❮ Previous
Next ❯

Citations

Nov 26, 2013·The Cancer Journal·Emilie GrossJack D Bui
Jan 19, 2016·Frontiers in Immunology·Raquel TarazonaRafael Solana
Jan 11, 2016·Journal of Internal Medicine·S MuenstS Hoeller
Dec 25, 2013·Immunologic Research·Richa Sharma, Asmita Das
Apr 30, 2014·European Journal of Immunology·Massimo VitaleLorenzo Moretta
Sep 1, 2015·Immunotherapy·Claudia CantoniMassimo Vitale
Aug 21, 2015·Cancer Immunology, Immunotherapy : CII·Gabriella PietraLorenzo Moretta
Mar 31, 2015·Pigment Cell & Melanoma Research·Andrea Ladányi
Aug 15, 2015·Stem Cells International·Pascal David Johann, Ingo Müller
Oct 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Korneel GrauwetHerman W Favoreel
Dec 25, 2012·Oncoimmunology·Mirna BalsamoMassimo Vitale
Nov 20, 2012·Oncoimmunology·Gabriella PietraMaria Cristina Mingari
Feb 13, 2015·Frontiers in Immunology·María Betina Pampena, Estrella Mariel Levy
Mar 19, 2014·Frontiers in Immunology·Lorenzo MorettaMaria Cristina Mingari
Oct 6, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhi-Hang YuanZhi-Liang Sun
Nov 29, 2016·Experimental Dermatology·Julia K TietzeCarola Berking
Aug 26, 2018·Journal of Cellular Physiology·Hassan Dianat-MoghadamMehdi Yousefi
Aug 16, 2019·Physiological Reviews·Carla Riera-DomingoMassimiliano Mazzone
Nov 5, 2019·Journal of Cellular Biochemistry·S Haleh HosseiniHamed Mohammadi
Apr 18, 2019·Journal of Cellular Physiology·Behnaz ValipourHojjatollah Nozad Charoudeh
Dec 28, 2019·Scandinavian Journal of Immunology·Costanza Maria CristianiEnnio Carbone
Sep 16, 2020·Journal of Leukocyte Biology·Claudia CantoniMassimo Vitale
Apr 5, 2019·Oncology Letters·Mihaela AntoheGabriela Turcu
Feb 20, 2019·Cellular & Molecular Immunology·Simona SivoriLorenzo Moretta
Apr 7, 2019·Cancer Immunology, Immunotherapy : CII·Beatriz Sanchez-CorreaRaquel Tarazona
Sep 25, 2019·International Journal of Molecular Sciences·Marie Frutoso, Erwan Mortier
Feb 18, 2020·Frontiers in Immunology·Nicola TuminoLorenzo Moretta
Mar 27, 2019·Cellular & Molecular Immunology·Guillaume HabifEmilie Narni-Mancinelli
Jan 2, 2019·Cancers·Seila Lorenzo-HerreroSegundo Gonzalez
Apr 11, 2020·Cancers·Christian Sordo-BahamondeSegundo Gonzalez
Jan 4, 2021·Critical Reviews in Oncology/hematology·Nobuyoshi Arima
Mar 26, 2021·Innate Immunity·Arosh Shavinda Perera Molligoda Arachchige
Apr 4, 2021·Cancers·Hansol LeeJames S Wilmott

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.