Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes

Scientific Reports
Maria Nathália MoraesAna Maria de Lauro Castrucci

Abstract

Melanopsin (OPN4) is a photo-pigment found in a small subset of intrinsically photosensitive ganglion cells (ipRGCs) of the mammalian retina. These cells play a role in synchronizing the central circadian pacemaker to the astronomical day by conveying information about ambient light to the hypothalamic suprachiasmatic nucleus, the site of the master clock. We evaluated the effect of a heat stimulus (39.5 °C) on clock gene (Per1 and Bmal1) expression in cultured murine Melan-a melanocytes synchronized by medium changes, and in B16-F10 melanoma cells, in the presence of the selective OPN4 antagonist AA92593, or after OPN4 knockdown by small interfering RNA (siRNA). In addition, we evaluated the effects of heat shock on the localization of melanopsin by immunocytochemistry. In both cell lines melanopsin was found in a region capping the nucleus and heat shock did not affect its location. The heat-induced increase of Per1 expression was inhibited when melanopsin was pharmacologically blocked by AA92593 as well as when its protein expression was suppressed by siRNA in both Melan-a and B16-F10 cells. These data strongly suggest that melanopsin is required for thermo-reception, acting as a thermo-opsin that ultimately feeds the local ...Continue Reading

References

Mar 15, 1987·International Journal of Cancer. Journal International Du Cancer·D C BennettI R Hart
Jan 1, 1993·Annual Review of Physiology·C S Pittendrigh
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 14, 2002·Science·Satchidananda PandaSteve A Kay
Jun 28, 2003·Science·Satchidananda PandaJohn B Hogenesch
May 15, 2010·Journal of Visualized Experiments : JoVE·Mirko Theis, Frank Buchholz
Oct 16, 2010·Science·Ethan D BuhrJoseph S Takahashi
Oct 22, 2010·The Journal of Investigative Dermatology·Florian SpörlAchim Kramer
Mar 12, 2011·Science·Wei L ShenCraig Montell
Apr 23, 2013·Handbook of Experimental Pharmacology·Steven A Brown, Abdelhalim Azzi
Aug 27, 2013·Nature Chemical Biology·Kenneth A JonesSatchidananda Panda
Jul 16, 2014·Blood Pressure Monitoring·Jacob RichardsMichelle L Gumz
Oct 7, 2014·Zoological Science·Akihisa Terakita, Takashi Nagata
Nov 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Gautam SikkaDan E Berkowitz
Nov 6, 2015·Scientific Reports·Serafín Pérez-CerezalesMichael Eisenbach
Aug 19, 2016·Molecular and Cellular Biochemistry·Maristela Oliveira PoletiniAna Maria de Lauro Castrucci
Nov 26, 2016·Science·Joseph Bass, Mitchell A Lazar
Dec 19, 2016·Nature Reviews. Genetics·Joseph S Takahashi
Feb 16, 2017·BMC Biology·Silke KiesslingNicolas Cermakian
Feb 17, 2017·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Leonardo Vinícius Monteiro de AssisAna Maria de Lauro Castrucci
May 23, 2017·Current Biology : CB·Annette E AllenRobert J Lucas
Jun 10, 2017·Annual Review of Cell and Developmental Biology·Nicole Y Leung, Craig Montell
Jul 12, 2017·Journal of Thermal Biology·Rodrigo JerônimoAna Maria de Lauro Castrucci

❮ Previous
Next ❯

Citations

Apr 4, 2018·International Journal of Molecular Sciences·Leonardo Vinícius Monteiro de AssisAna Maria de Lauro Castrucci
Sep 1, 2018·Lasers in Surgery and Medicine·Irene Castellano-PellicenaM Julie Thornton
Aug 14, 2018·Photochemistry and Photobiology·Anna Stachurska, Tadeusz Sarna
Jun 22, 2019·Cellular and Molecular Life Sciences : CMLS·Leonardo Vinícius Monteiro de AssisAna Maria de Lauro Castrucci
Jun 28, 2018·Frontiers in Oncology·Leonardo Vinícius Monteiro de AssisAna Maria de Lauro Castrucci
Mar 7, 2020·Science·Evelina SjöstedtJan Mulder
Mar 9, 2021·Cellular and Molecular Life Sciences : CMLS·Leonardo Vinícius Monteiro de Assis, Henrik Oster
Jul 9, 2021·Cell and Tissue Research·Maria Nathalia MoraesAna Maria de Lauro Castrucci
Jul 10, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Leonardo Vinícius Monteiro de AssisAna Maria de Lauro Castrucci

❮ Previous
Next ❯

Methods Mentioned

BETA
gene knockdown
transfection

Software Mentioned

Axiovision
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.