PMID: 8943000Nov 26, 1996Paper

Melanotropic peptide-conjugated beads for microscopic visualization and characterization of melanoma melanotropin receptors

Proceedings of the National Academy of Sciences of the United States of America
S D SharmaV J Hruby

Abstract

We developed two solid-phase reagent systems for microscopic visualization and characterization of melanocyte-stimulating hormone (MSH) receptors of melanoma cells. Multiple copies of [Nle-4,D-Phe-7]-alpha-MSH, a potent analog of alpha-MSH, were conjugated to microspheres (latex beads) or macrospheres (polyamide beads) through a thioether or disulfide bond. Binding between the beads and mouse and human melanoma cells was examined by scanning electron microscopy and by light microscopy. Each mouse and human melanoma cell (of all cell lines) evinced binding to the beads. Binding of the melanotropin conjugates was not restricted to any one phase of the cell cycle. Specificity of binding was demonstrated by several studies. Negative controls included cell types of nonmelanocyte origin (e.g., mammary cancer cells) and beads that lacked the melanotropic ligand or had other attached ligands. Beads with a disulfide-linked melanotropin analog served as a direct control. Treatment of these beads with DTT during or before incubation of the beads with melanoma cells (resulting in release of the MSH analog from the beads) eliminated binding of the beads to melanoma cells. Binding interactions between melanoma cells and melanotropin-bound be...Continue Reading

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Citations

Jan 22, 2008·European Journal of Nuclear Medicine and Molecular Imaging·Zi-Bo LiXiaoyuan Chen
Mar 17, 2010·Amino Acids·Yongjun Yan, Xiaoyuan Chen
Dec 20, 2003·Bioorganic & Medicinal Chemistry Letters·Josef VagnerVictor J Hruby
Jun 7, 2011·Bioconjugate Chemistry·Jatinder S JosanRobert J Gillies
Aug 13, 2010·The Journal of Organic Chemistry·Ramesh AlletiEugene A Mash
Dec 17, 2009·The Journal of Organic Chemistry·Victor J Hruby
Aug 13, 2009·Molecular Cancer Therapeutics·Liping XuRobert J Gillies
Jan 9, 2013·Annual Review of Pharmacology and Toxicology·Victor J Hruby, Minying Cai
Aug 29, 2009·International Journal of Peptide Research and Therapeutics·Jatinder S JosanVictor J Hruby
Feb 15, 2011·European Journal of Pharmacology·Minying CaiVictor J Hruby
Jan 20, 2006·Pigment Cell Research·Donald W RobertsRichard A Sturm
Dec 9, 2004·Expert Opinion on Therapeutic Targets·Heather L HandlRobert J Gillies
Apr 1, 1999·FEBS Letters·S R BarbosaC R Nakaie
May 19, 2000·Annals of the New York Academy of Sciences·S Solomon
Apr 24, 2007·Bioorganic & Medicinal Chemistry Letters·Bhumasamudram JagadishEugene A Mash

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