Melatonin activates FIS1, DYN1, and DYN2 Plasmodium falciparum related-genes for mitochondria fission: Mitoemerald-GFP as a tool to visualize mitochondria structure

Journal of Pineal Research
Pedro H ScarpelliCelia R S Garcia

Abstract

Malaria causes millions of deaths worldwide and is considered a huge burden to underdeveloped countries. The number of cases with resistance to all antimalarials is continuously increasing, making the identification of novel drugs a very urgent necessity. A potentially very interesting target for novel therapeutic intervention is the parasite mitochondrion. In this work, we studied in Plasmodium falciparum 3 genes coding for proteins homologues of the mammalian FIS1 (Mitochondrial Fission Protein 1) and DRP1 (Dynamin Related Protein 1) involved in mitochondrial fission. We studied the expression of P. falciparum genes that show ample sequence and structural homologies with the mammalian counterparts, namely FIS1, DYN1, and DYN2. The encoded proteins are characterized by a distinct pattern of expression throughout the erythrocytic cycle of P. falciparum, and their mRNAs are modulated by treating the parasite with the host hormone melatonin. We have previously reported that the knockout of the Plasmodium gene that codes for protein kinase 7 is essential for melatonin sensing. We here show that PfPk7 knockout results in major alterations of mitochondrial fission genes expression when compared to wild-type parasites, and no change ...Continue Reading

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Citations

Aug 9, 2019·Journal of Pineal Research
Jan 28, 2021·Pharmaceutics·Dalia M Kopustinskiene, Jurga Bernatoniene
Jan 21, 2021·International Journal of Molecular Sciences·Pedro H ScarpelliCelia R S Garcia
Aug 17, 2021·Frontiers in Cellular and Infection Microbiology·Lucas Silva de OliveiraSébastien Charneau
Aug 25, 2021·Current Opinion in Microbiology·Benedito M Dos SantosCélia Rs Garcia

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Methods Mentioned

BETA
GTPase
ubiquitination
PCR
electrophoresis
transfection
light microscopy

Software Mentioned

Matrix EBLOSUM62
BLAST
AxioVision
EMBOSS Needle
GraphPad Prim

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Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.