Melatonin acts as antioxidant and pro-oxidant in an organotypic slice culture model of Alzheimer's disease

Neuroreport
Kimberly L Clapp-LillyLawrence K Duffy

Abstract

An organotypic mouse brain slice culture system of Alzheimer's disease (AD) under low oxygen partial pressures was developed to determine the antioxidant properties of the pineal hormone melatonin in vitro. Assays for biochemical markers of oxidative stress including redox active iron assay, heme-oxygenase-1 and 8-hydroxyguanosine inmunoreactivity were performed along with morphological analysis for stressed tissue following amyloid-beta (A beta) 1-40 insult. Melatonin (100 microM) significantly reduced the appearance of condensed chromatin, redox active iron, heme-oxygenase-1 induction and 8-hydroxyguanosine immunoreactivity caused by 50 microM A beta. Melatonin also prevented A beta-induced morphological signs of oxidative stress in tissue ultrastructure, including edema and dark degenerating profiles as visualized under electron microscope. At elevated concentrations (1 mM), melatonin induced redox active iron and heme-oxgenase-1 immunoreactivity. Thus, while melatonin may be a potential therapeutic agent in the prevention of oxidative stress associated with A beta and AD, it can also induce markers of oxidative stress at millimolar concentrations.

References

May 1, 1995·Neurochemistry International·L R Barlow-WaldenB Poeggeler
Oct 1, 1995·The Biochemical Journal·L L IversenM S Shearman
May 28, 1993·Annals of the New York Academy of Sciences·M P MattsonV L Smith-Swintosky
Jul 11, 1996·Nature·A FriebeD Koesling
Jul 11, 1996·Nature·M A SmithN Kowall
Jan 1, 1997·Free Radical Biology & Medicine·W R Markesbery
Jan 4, 1998·Journal of Molecular Neuroscience : MN·W Song, D K Lahiri
Apr 29, 1998·The Journal of Biological Chemistry·M PappollaJ Ghiso
Nov 24, 1998·Biochemical Pharmacology·C E BeyerD Saphier
Feb 15, 2000·Annals of the New York Academy of Sciences·D K Lahiri, C Ghosh
Feb 15, 2000·Annals of the New York Academy of Sciences·A NunomuraM A Smith

❮ Previous
Next ❯

Citations

Oct 22, 2003·Physiology & Behavior·K AngersL Thibault
Feb 9, 2010·Antioxidants & Redox Signaling·Carlotta GiorgiPaolo Pinton
Jul 24, 2010·Antioxidants & Redox Signaling·Cynthia A Massaad, Eric Klann
Oct 22, 2002·Expert Opinion on Investigational Drugs·Bernd Moosmann, Christian Behl
Jul 29, 2009·Critical Reviews in Biochemistry and Molecular Biology·Russel J ReiterDan-Xian Tan
Nov 12, 2013·The American Journal of Geriatric Psychiatry : Official Journal of the American Association for Geriatric Psychiatry·José R Maldonado
May 23, 2009·Toxicology and Applied Pharmacology·F RadognaL Ghibelli
May 26, 2009·Toxicology and Applied Pharmacology·Flavia RadognaLina Ghibelli
Mar 13, 2009·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Zoreh Davanipour, Eugene Sobel
Mar 24, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kriya L DunlapLawrence K Duffy
Apr 1, 2005·International Journal of Cancer. Journal International Du Cancer·Uwe WenzelHannelore Daniel
Jan 15, 2009·Journal of Pineal Research·Małgorzata M PosmykK M Janas
Aug 2, 2006·Journal of Pineal Research·M Rosa NoguésJordi Mallol
Jul 26, 2014·Journal of Pineal Research·Hong-Mei Zhang, Yiqiang Zhang
Aug 9, 2015·Neuroscience·C Humpel
May 9, 2006·Biochimica Et Biophysica Acta·Vladimir N AnisimovIrina A Vinogradova
Sep 28, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Albert WölflerPeter M Liebmann
Oct 31, 2003·Toxicologic Pathology·Vladimir N Anisimov
Nov 5, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sara M BeniEsther Shohami
Sep 11, 2019·Journal of Clinical Medicine·Farhad Md HossainYonggeun Hong
Aug 25, 2020·Oxidative Medicine and Cellular Longevity·Alejandra Guillermina Miranda-DíazErnesto Germán Cardona-Muñoz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.