Melatonin alleviates oxidative stress-inhibited osteogenesis of human bone marrow-derived mesenchymal stem cells through AMPK activation

International Journal of Medical Sciences
Sooho LeeDongchul Kang

Abstract

Oxidative stress plays an important role in the pathogenesis of aging-related osteoporosis through the increased bone resorption or reduced bone formation. Melatonin, which can exert beneficial actions through antioxidant, anti-inflammatory, and bone-preserving effects, shows promise in preventing oxidative stress-inhibited osteogenesis. However, specific mechanisms by which melatonin rescues oxidative stress-inhibited osteogenesis of human mesenchymal stem cells (MSCs) have not been fully elucidated yet. We therefore investigated whether activation of AMPK by melatonin regulates the antagonistic crosstalk between oxidative stress and osteogenic differentiation in human MSCs. Melatonin treatment significantly enhanced osteogenic differentiation of human MSCs through activation of AMPK and upregulation of FOXO3a and RUNX2 which were known as master transcription factors responsible for the mechanistic link between oxidative stress and osteogenic phenotype. Osteogenic differentiation determined by calcium deposition was significantly increased by melatonin treatment against oxidative stress. In addition, melatonin treatment reconstituted activation of AMPK and expression of FOXO3a and RUNX2 inhibited by oxidative stress. Overall,...Continue Reading

Citations

Oct 22, 2019·Journal of Pineal Research·Nan FangLifeng Liu
Dec 7, 2019·Toxicology Mechanisms and Methods·Latifa KnaniSergio Minucci
Sep 17, 2019·World Journal of Stem Cells·Sara CrucianiMargherita Maioli
Aug 23, 2019·Annual Review of Nutrition·Xuan YangZhenglong Gu
May 1, 2021·Materials·Cristina ChircovAlexandru Mihai Grumezescu
Jun 5, 2021·Journal of Pineal Research·Fahima Munmun, Paula A Witt-Enderby
Nov 20, 2021·Frontiers in Pharmacology·Haozhe YuYun Feng

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