Melatonin ameliorates TNBS-induced colitis in rats through the melatonin receptors: involvement of TLR4/MyD88/NF-κB signalling pathway

Inflammopharmacology
Mohsen ChamanaraSeyed-Mahdi Rezayat

Abstract

The aim of the present study is to investigate the anti-inflammatory effect of melatonin in trinitrobenzene sulfonic acid (TNBS)-induced rat colitis through the inhibition of Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) signalling pathway and activation of melatonin receptor. Colitis was induced in Wistar rats by administration of 100 mg/kg TNBS dissolved in 0.25 ml of 50% ethanol solution using a flexible plastic rubber catheter into the colon via the anus. This resulted in incidence of colitis on the first day, and all treatments were conducted for 10 days after induction of colitis. Melatonin was administered intraperitoneally (i.p.) at doses of 1, 5, and 10 mg/kg/day. Luzindole (non-selective MT1/MT2 receptor antagonist) was administered i.p. at dose of 5 mg/kg/day 15 min prior to melatonin injection. During the experiment, animals were monitored for the appearance of diarrhoea, body weight loss, and rectal bleeding. Myeloid peroxidase enzyme and tumour necrosis factor-α (TNF-α) activities were detected by immunohistochemistry. The protein expression level of TLR4, myeloid differentiation factor 88 (MyD88), NF-κB p65, and inhibitor of kappa B (I-κB) were detected by western blotting analysis. Treatment with me...Continue Reading

References

Mar 5, 2002·Nature Immunology·Michael Karin, Anning Lin
Jun 12, 2003·World Journal of Gastroenterology : WJG·Wei-Guo DongYu Xu
Feb 4, 2005·Journal of Leukocyte Biology·Seymour J Klebanoff
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Madeline M FortRobert M Hershberg
Oct 4, 2005·The Journal of Clinical Investigation·Jun-Li LuoMichael Karin
May 16, 2008·Journal of Internal Medicine·I AtreyaM F Neurath
Apr 11, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ryan UngaroMaria T Abreu
Jul 16, 2010·The Journal of Pharmacology and Experimental Therapeutics·Jie ChengFrank J Gonzalez
Jan 12, 2011·Digestive Diseases and Sciences·Iqbal Siddique, Islam Khan
Dec 14, 2011·Fundamental & Clinical Pharmacology·Seda TasdemirAhmet Acet
May 7, 2013·Journal of Molecular and Cellular Cardiology·Ji-Youn YounHua Cai
Jun 4, 2013·International Journal of Molecular Sciences·Gu-Jiun LinHuey-Kang Sytwu
Oct 22, 2013·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xia YangHong-Yu Ren
Feb 21, 2014·Cellular and Molecular Life Sciences : CMLS·Darío Acuña-CastroviejoRussel J Reiter
Jun 13, 2014·Cellular and Molecular Life Sciences : CMLS·Jerry Vriend, Russel J Reiter
Sep 16, 2014·Life Sciences·Jerry Vriend, Russel J Reiter
May 16, 2015·Canadian Journal of Physiology and Pharmacology·Sattar OstadhadiAhmad-Reza Dehpour
Jan 15, 2016·Canadian Journal of Physiology and Pharmacology·Saeed MehrzadiHabib Ghaznavi
Apr 4, 2016·Inflammopharmacology·Amir RashidianSeyed-Mahdi Rezayat
Oct 26, 2016·Journal of Pineal Research·Azam HosseinzadehSaeed Mehrzadi
Apr 26, 2017·Journal of Pineal Research·Maryam MajidiniaBahman Yousefi
Jun 28, 2017·Journal of Inflammation Research·Erin R LaneDavid L Suskind

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Citations

Jul 28, 2019·Journal of Pineal Research·Giuseppe ManninoCarla Gentile
Nov 30, 2019·Innate Immunity·Yisheng LuoLi Zhang
Jul 15, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jinghui YangLiming Wang
Sep 19, 2020·International Journal of Molecular Sciences·David DahlgrenHans Lennernäs
Oct 31, 2020·Current Medical Science·Juan LiGuang Du
May 22, 2021·Frontiers in Pharmacology·David DahlgrenHans Lennernäs
Jul 14, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Cailan LiQiang Lu
Jul 17, 2021·Acta Biomaterialia·Xiangbing WuHongchang Lai
Oct 3, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mojgan MorvaridzadehJavad Heshmati
Aug 29, 2021·Pharmaceuticals·Shijia PanYonggong Zhai

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