PMID: 9546044Apr 18, 1998Paper

Melatonin and serotonin interactions with calmodulin: NMR, spectroscopic and biochemical studies

Biochimica Et Biophysica Acta
H Ouyang, Hans J Vogel

Abstract

It has been reported that the hormone melatonin binds tightly to the ubiquitous calcium-regulatory protein, calmodulin (CaM) with a Kd value around 0.1 nM [Benítez-King et al., Biochim. Biophys. Acta, 1290 (1993) 191-196]. Normally CaM only binds to target proteins and various 20-residue synthetic peptides encompassing the CaM-binding domain of these target proteins with K(d) values ranging between 1.0 microM and 0.1 nM. Here we have studied the interaction of melatonin and several structurally related compounds--serotonin, 5-hydroxytryptophan, and tryptophan--to CaM through gel band shift assays, enzymatic competition assays with calcineurin, fluorescence spectroscopy, far and near UV circular dichroism spectropolarimetry and NMR spectroscopy. Fluorescence spectra show that the binding is calcium dependent. NMR studies with biosynthetically labelled methyl-13C-Met CaM show that melatonin and the other compounds interact with the hydrophobic cleft regions of the protein. Our NMR data show that melatonin binds to both domains of the dumbbell-shaped CaM, while serotonin appears to bind only to the C-terminal domain. This binding mode is further substantiated by fluorescence and gel band shift competition experiments with syntheti...Continue Reading

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Citations

Sep 27, 2008·Journal of Biomolecular NMR·Sherwin J AbrahamVadim Gaponenko
Oct 27, 1999·Journal of Pineal Research·Y NodaL Packer
Oct 23, 2004·Protein Science : a Publication of the Protein Society·Adrián G TurjanskiVincenzo Martorana
Sep 5, 2006·Journal of Pineal Research·Eduardo K TamuraRegina P Markus
Jul 13, 2016·Molecular and Cellular Endocrinology·Ricardo Letra-VilelaFederico Herrera
Mar 15, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·June Hyun HanKyung Do Kim
Dec 24, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Ling YangXiaoying Zhang
Jun 13, 2014·Cellular and Molecular Life Sciences : CMLS·Jerry Vriend, Russel J Reiter
Feb 26, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alziana Moreno da Cunha PedrosaGustavo Pessini Amarante-Mendes
Jul 7, 2011·Biopolymers·Asghar Taheri-KafraniThomas Haertlé
Nov 5, 2014·Journal of Pineal Research·Jerry Vriend, Russel J Reiter
Dec 6, 2019·Frontiers in Endocrinology·Lei LiuRalf Jockers
Nov 17, 2020·Frontiers in Chemistry·Falguni ChandraApurba L Koner
Dec 18, 2020·Journal of Pineal Research·Erika CeconRalf Jockers

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