Melatonin attenuated adipogenesis through reduction of the CCAAT/enhancer binding protein beta by regulating the glycogen synthase 3 beta in human mesenchymal stem cells

Journal of Physiology and Biochemistry
Yun-Hee Rhee, Jin-Chul Ahn

Abstract

Adipogenic differentiation is characterized by an increase in two major transcription factors: peroxisome proliferator-activated receptor gamma (PPARγ) and the CCAAT/enhancer binding protein alpha (C/EBPα). These two signals are influenced by C/EBPβ and C/EBPδ and cross-regulate each other's expression during the initial stages of adipogenesis. Melatonin has been known to act as not only a direct scavenger of free radicals but also an inhibitor of glycogen synthase kinase 3β (GSK-3β). Here, we report that melatonin inhibits the adipogenic differentiation of human mesenchymal stem cells (hMSCs) which is due to the regulations of C/EBPβ in the early stage of adipogenic differentiation. Melatonin reduced the lipid accumulation, adiponectin, and lipoprotein lipase (LPL) during the adipogenic differentiation of hMSCs. Since C/EBPβ has been associated with the activation of PPARγ and the consensus site of ERK/GSK-3β, PPARγ and β-catenin were detected by immunofluorescence staining after pretreatment of melatonin. Melatonin blocked the activation of PPARγ which induced the degradation of β-catenin. Melatonin also decreased the levels of cyclic adenosine-3,5-monophosphate (cAMP) and reactive oxygen species (ROS). The cAMP triggered the...Continue Reading

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Citations

Dec 22, 2016·International Journal of Molecular Sciences·Dun-Xian TanRussel J Reiter
Aug 27, 2018·Journal of Cellular Physiology·Biao WangLingbo Kong
Jan 4, 2019·Animal Biotechnology·Yan WangLinjie Wang
Jul 30, 2019·Advances in Nutrition·Ziye XuTizhong Shan
Apr 2, 2020·Cells·Leonie HoffmeisterRené Huber
Aug 30, 2018·International Journal of Endocrinology·Francisco J Valenzuela-MelgarejoGerardo Cabello-Guzmán
Oct 15, 2019·Frontiers in Nutrition·Marius FarcasIoana Berindan-Neagoe

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