Melatonin attenuates chronic cough mediated by oxidative stress via transient receptor potential melastatin-2 in guinea pigs exposed to particulate matter 2.5

Physiological Research
Zhenjun JiKefang Lai

Abstract

The aim of this study was to investigate the effects of melatonin on oxidative stress, the expression of transient receptor potential melastatin-2 (TRPM2) in guinea pig brains, and the influence of melatonin on oxidative stress in lungs and airway inflammation induced by particulate matter 2.5 (PM2.5). A particle suspension (0.1 g/ml) was nasally administered to the guinea pigs to prepare a PM2.5 exposure model. Cough frequency and cough incubation period were determined through RM6240B biological signal collection and disposal system. Oxidative stress markers, including malondialdehyde (MDA), total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-Px), in the medulla oblongata were examined through spectrophotometer. Reactive oxygen species (ROS) were detected in the hypoglossal nucleus, cuneate nucleus, Botzinger complex, dorsal vagal complex, and airway through dihydroethidium fluorescence. Hematoxylin-eosin (HE) staining and substance P expression via immunohistochemistry revealed the inflammatory levels in the airway. TRPM2 was observed in the medulla oblongata through immunofluorescence and Western blot. The ultrastructure of the blood-brain barrier and neuronal mitochondria...Continue Reading

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Citations

Feb 6, 2019·The British Journal of Psychiatry : the Journal of Mental Science·Xuelin GuShaowei Wu
Apr 2, 2020·Physiological Research·M LucanskaR Pecova
Apr 2, 2020·Physiological Research·M Brozmanova, N Pavelkova
Jan 23, 2021·Environmental Science and Pollution Research International·Qisijing LiuShaowei Wu
Apr 4, 2021·International Journal of Molecular Sciences·Alina Milici, Karel Talavera
Dec 2, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuanming LiJinhao Sun
May 17, 2021·Pharmacology & Therapeutics·Ankit AryalTammy R Dugas
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Yue HaoXianhong Gu

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