Melatonin attenuates osteosarcoma cell invasion by suppression of C-C motif chemokine ligand 24 through inhibition of the c-Jun N-terminal kinase pathway

Journal of Pineal Research
Ko-Hsiu LuShun-Fa Yang

Abstract

Osteosarcoma, with its high metastatic potential, is the most prevalent malignant bone tumor in children and adolescents. Melatonin possesses multiple tumor-suppressing properties for a myriad of tumors, but little is known about the effects of melatonin on osteosarcoma metastasis. In this study, we demonstrated that melatonin elicited very low cytotoxicity and significantly inhibited cellular motility, migration, and invasion in human osteosarcoma U2OS and HOS cells. Moreover, using RNA sequencing technology, we revealed that melatonin repressed C-C motif chemokine ligand 24 (CCL24) gene expression in U2OS cells. Manipulation of CCL24 levels influenced the motility of osteosarcoma cells as cell migration and invasion were enhanced by the addition of recombinant human CCL24 and attenuated by the silencing of CCL24. Moreover, melatonin increased and decreased the activation of extracellular signal-regulated kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK) 1/2, respectively, in a dose-dependent manner in U2OS and HOS cells while exerting no evident influence on the level and activation of p38, Akt, FAK, steroid receptor coactivator, or Raf. In further functional experiments, the use of JNK inhibitors (SP600125 and DN-JNK) confi...Continue Reading

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Citations

Apr 5, 2019·Journal of Cellular Physiology·Yu-Hua ChaoShun-Fa Yang
Mar 19, 2019·Frontiers in Endocrinology·Consolato SergiSong-Mei Liu
Dec 5, 2019·Cancer Cell International·Hadis FathizadehZatollah Asemi
Sep 19, 2020·Cancer Cell International·Mohammad Hossein MehrzadiSaeed Mehrzadi
Feb 1, 2019·Scientific Reports·Bruna Victorasso Jardim-PerassiDebora Aparecida Pires de Campos Zuccari
Dec 18, 2019·Cells·Ko-Hsiu LuShun-Fa Yang
Dec 23, 2020·Epigenomics·Mohammad Hossein PourhanifehAzam Hosseinzadeh
Dec 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ko-Hsiu LuJia-Sin Yang
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haozhen MaFang Huang
Jan 20, 2020·Molecular Therapy. Nucleic Acids·Peng LiuXiaoming Xie

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