Melatonin diminishes oxidative damage in sperm cells, improving assisted reproductive techniques

Turkish Journal of Biology = Türk Biyoloji Dergisi
Fabian MonllorIgnacio Bejarano

Abstract

Sperm preparation procedures are a potential generator of oxidative stress-induced DNA damage, which leads to a dramatic drop in fertility. An increasing number of studies suggest that melatonin reduces the oxidative stress induced by manipulation. However, very little is known about the preservative role of melatonin in sperm preparation medium during assisted reproduction procedures. For this aim to be achieved, semen was divided into two fractions and preincubated with and without 1 mM melatonin. Afterwards, both fractions were divided into two subfractions to perform swim-up in the presence and absence of 1 mM melatonin. Labeling with anti-CD46 and antiactive caspase-3 allowed the monitoring of acrosome reaction and apoptosis by flow cytometry. Sperm DNA fragmentation and compaction were analyzed through propidium iodide staining. The normozoospermic and oligozoospermic samples that were preincubated with melatonin underwent a significant increase in the ratio of adequate spermatozoa and a reduction of caspase-3 activation. Additionally, preincubation with melatonin enhanced the migration of sperm cells with compacted DNA in oligozoospermic samples (P < 0.05) and prevented DNA fragmentation in normozoospermic samples (P < 0...Continue Reading

Citations

Jul 14, 2018·Frontiers in Cell and Developmental Biology·Ignacio BejaranoJosé A Pariente
Sep 2, 2020·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Naina Kumar, Namit Kant Singh

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