Melatonin-induced autophagy is associated with degradation of MyoD protein in C2C12 myoblast cells

Journal of Pineal Research
Chi Hyun KimYeong-Min Yoo

Abstract

MyoD is a muscle-specific transcriptional factor that acts as a master switch for skeletal muscle differentiation. This protein regulates myoblast proliferation and myogenic differentiation and is also a short-lived regulatory protein that is degraded by the ubiquitin system. However, the lysosomal pathway of MyoD protein degradation remains unknown. In this study, we sought to determine whether melatonin (1, 2mm)-induced autophagy causes the degradation of MyoD protein in C2C12 myoblast cells. Melatonin induced a significant increase in expression of the microtubule-associated protein 1 light chain 3 (LC3)-II and Beclin-1 proteins in a dose-dependent manner. Melatonin treatment also significantly increased p-ERK, Ras, and p-Akt expressions in a dose-dependent manner. However, Bax expression was high compared with the absence of melatonin treatment, and Bcl-2 expression was high in the 0.1-0.5mm melatonin treatments and low in the 1 and 2mm melatonin treatments. Under the same conditions, cytosolic MyoD protein was significantly decreased in a dose-dependent manner and completely eliminated by 36hr. This decrease in MyoD protein involved ubiquitin-mediated proteasomal activity with proteasome inhibitor MG132 or autophagy-depend...Continue Reading

References

Oct 7, 1994·Cell·A Ciechanover
May 15, 1996·Genes & Development·L A MegeneyM A Rudnicki
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser
Aug 26, 1998·Molecular and Cellular Biology·A SongM A Harrington
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·N F Gonzalez-CadavidS Bhasin
Oct 20, 1999·Domestic Animal Endocrinology·J BassR Kambadur
Jan 8, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M WehlingJ G Tidball
Feb 13, 2001·American Journal of Physiology. Endocrinology and Metabolism·W E TaylorN Gonzalez-Cadavid
Feb 13, 2001·Biochemical and Biophysical Research Communications·R RíosJ Devesa
Apr 20, 2001·The Journal of Biological Chemistry·Z E FloydA L Schwartz
Aug 23, 2001·Current Opinion in Clinical Nutrition and Metabolic Care·R T Jagoe, A L Goldberg
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·M D GomesA L Goldberg
Oct 25, 2002·The Journal of Biological Chemistry·Jody M LingbeckAlan L Schwartz
Dec 9, 2003·The Journal of Biological Chemistry·Sabrina BatonnetSerge Alexandre Leibovitch
Dec 17, 2003·Journal of Pineal Research·Carmen RodriguezRussel J Reiter
Apr 16, 2004·American Journal of Physiology. Cell Physiology·Minenori IshidoMitsuhiko Masuhara
Apr 23, 2004·Trends in Cell Biology·Ana Maria Cuervo
Nov 6, 2004·Science·Takahiro Shintani, Daniel J Klionsky
Nov 9, 2004·The Journal of Biological Chemistry·Lionel A TintignacSerge A Leibovitch
Sep 9, 2005·Nature Reviews. Cancer·Yasuko KondoSeiji Kondo
Oct 26, 2005·Cell Death and Differentiation·T Yorimitsu, D J Klionsky
Oct 4, 2006·Experimental Cell Research·Sabrina Batonnet-PichonSerge A Leibovitch
Aug 19, 2007·Molecular Biology of the Cell·Sean M KellyLinda S Musil
Dec 7, 2007·Cell Metabolism·Cristina MammucariMarco Sandri

❮ Previous
Next ❯

Citations

Oct 27, 2016·International Journal of Molecular Sciences·Ana Coto-MontesRussel J Reiter
Feb 28, 2015·The Journal of Biological Chemistry·Dechen FuNadine Wiper-Bergeron
Mar 12, 2020·Journal of Muscle Research and Cell Motility·Bide ChenTizhong Shan
Mar 24, 2021·Experimental Gerontology·Hongfu JinYusheng Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms