Melatonin induces anti-inflammatory effects via endoplasmic reticulum stress in RAW264.7 macrophages.

Molecular Medicine Reports
Yina ChenJiansheng Wu

Abstract

Melatonin, which is predominantly secreted by the pineal gland and is released into the blood, appears to have anti‑inflammatory properties. Several studies have shown that melatonin can relieve lipopolysaccharide-induced inflammatory responses of RAW264.7 cells. However, the mechanisms underlying this anti‑inflammatory effect remain to be fully elucidated, particularly the association between melatonin and endoplasmic reticulum (ER) stress (ERS). Therefore, the present study examined the anti‑inflammatory activity of melatonin in RAW264.7 cells and analyzed its molecular mechanisms in ERS. The RAW264.7 cells were stimulated by lipopolysaccharide and treated with melatonin. A Cell Counting Kit‑8 assay was used to assess the toxicity of melatonin. The degree of inflammation was evaluated using ELISA. The expression levels of ERS‑associated protein molecules were examined using reverse transcription‑quantitative polymerase chain reaction and western blot analyses. The results revealed that melatonin had no toxic effect on the RAW264.7 cells at the range of concentrations used in the experiment. Lipo-polysaccharide stimulated the cells to produce inflammatory molecules; in the early stage, proteins associated with ERS increased, a...Continue Reading

References

Aug 16, 2001·Trends in Biochemical Sciences·A S Lee
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
Jan 20, 2004·Trends in Cell Biology·D Thomas Rutkowski, Randal J Kaufman
Sep 15, 2004·Journal of Chemical Neuroanatomy·Xiaohua ShenRandal J Kaufman
Jan 7, 2006·Cell Death and Differentiation·M Boyce, J Yuan
Nov 27, 2007·Cellular and Molecular Life Sciences : CMLS·M Schröder
Dec 25, 2007·Trends in Endocrinology and Metabolism : TEM·Andrzej SlominskiRalf Paus
Jan 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kunihiro HayakawaMasanori Kitamura
Aug 27, 2010·Journal of Pineal Research·Katherine FormanJ A F Tresguerres
Sep 8, 2011·Investigative Ophthalmology & Visual Science·Daniella do Carmo BuonfiglioJosé Cipolla-Neto
Dec 30, 2011·European Journal of Pediatrics·Anat Cohen EnglerGiora Pillar
Feb 9, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·A CarpentieriN Tolosa de Talamoni
Aug 14, 2012·Trends in Molecular Medicine·Abhishek D GargPatrizia Agostinis

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