Melatonin induces calcium mobilization and influences cell proliferation independently of MT1/MT2 receptor activation in rat pancreatic stellate cells

Cell Biology and Toxicology
Patricia Santofimia-CastañoAntonio Gonzalez

Abstract

Melatonin, the product of the pineal gland, possesses antioxidant, anti-inflammatory, and antitumor properties in different tissues, in addition to its role as regulator of biological rhythms. In this study, the effects of pharmacological concentrations of melatonin (1 μM-1 mM) on pancreatic stellate cells (PSCs) have been examined. Cell viability was studied using AlamarBlue® test. Cell-type specific markers and total amylase content were analyzed by immunocytochemistry and colorimetric methods, respectively. Changes in intracellular free Ca(2+) concentration were followed by fluorimetric analysis of fura-2-loaded cells. The cellular red-ox state was monitored following CM-H2DCFDA-derived fluorescence. Determination of the activation of p44/42 mitogen-activated protein kinase (MAPK), SAPK/JNK and p38 was measured by Western blot analysis. Our results show that PSCs viability decreased in the presence of 100 μM or 1 mM melatonin. However, in the presence of 1 or 10 μM melatonin, no changes in cell viability were observed. Melatonin MT1 and MT2 receptors could not be detected. Melatonin induced Ca(2+) mobilization from intracellular pools. In the presence of melatonin, activation of crucial components of MAPKs pathway was notice...Continue Reading

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Citations

Apr 17, 2016·Journal of Cellular and Molecular Medicine·Diane C WangChengshui Chen
Oct 8, 2016·Cell Biology and Toxicology·Madhav Bhatia
Jun 27, 2018·Journal of Cellular Physiology·Kuo-Feng HuangSheau-Huei Chueh
Apr 15, 2020·Scientific Reports·Antonio GonzalezGines M Salido
Jul 1, 2015·Current Opinion in Gastroenterology·Minote ApteJeremy S Wilson
Jul 8, 2020·Biology of the Cell·Matias EstarasAntonio Gonzalez
May 19, 2020·Journal of Pineal Research·Hyocheol BaeWhasun Lim

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