Melatonin-Mediated Development of Ovine Cumulus Cells, Perhaps by Regulation of DNA Methylation

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Yi FangYixun Liu

Abstract

Cumulus cells of pre-pubertal domestic animals are dysfunctional, perhaps due to age-specific epigenetic events. This study was designed to determine effects of melatonin treatment of donors on methylation modification of pre-pubertal cumulus cells. Cumulus cells from germinal vesicle stage cumulus oocyte complexes (COCs) were collected from eighteen lambs which were randomly divided into control group (C) and melatonin group given an 18 mg melatonin implant subcutaneous (M). Compared to the C group, the M group had higher concentrations of melatonin in plasma and follicular fluid (p < 0.05), greater superovulation, a higher proportion of fully expanded COCs, and a lower proportion of apoptotic cumulus cells (p < 0.05). Real-time PCR results showed that melatonin up-regulated expression of genes MT1, Bcl2, DNMT1, DNMT3a and DNMT3b, but down-regulated expression of genes p53, Caspase 3 and Bax (p < 0.05). Furthermore, melatonin increased FI of FITC (global methylation level) on cumulus cells (p < 0.05). To understand the regulation mechanism, the DNMTs promoter methylation sequence were analyzed. Compared to the C group, although there was less methylation at two CpG sites of DNMT1 (p < 0.05) and higher methylation at two CpG si...Continue Reading

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Citations

Feb 23, 2019·Animal Science Journal = Nihon Chikusan Gakkaihō·Hasan RiazLiguo Yang
Jun 20, 2019·International Journal of Molecular Sciences·Marwa El SheikhIl-Keun Kong
Feb 13, 2020·International Journal of Molecular Sciences·Hiroshi TamuraNorihiro Sugino
Mar 14, 2021·Reproductive Sciences·Zhixin Jiang, Huan Shen
Apr 18, 2021·Molecular and Cellular Biochemistry·Maryam EzzatiRaziyeh Kheirjou

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Methods Mentioned

BETA
acetylation
enzyme-linked immunosorbent assay
ELISA
flow cytometry
PCR

Software Mentioned

SuperCyt Analyst
ASW
FV10
FACS Analyzer
SPSS

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