Melatonin prevents oxidized low-density lipoprotein-induced increase of myosin light chain kinase activation and expression in HUVEC through ERK/MAPK signal transduction

Journal of Pineal Research
Hua-Qing ZhuYuan Wang

Abstract

Melatonin, the main secretary product of the pineal gland, is potentially effective in the prevention of a number of diseases in which free radical processes are involved. The development of hypercholesterolemia is a multifactorial process in which elevated oxidized low-density lipoprotein (ox-LDL) levels play a central role. The purpose of this study was to test whether melatonin prevents ox-LDL-induced increase of myosin light chain kinase (MLCK) activation and expression in human umbilical vein endothelial cells (HUVECs) through extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) signal transduction. HUVEC were cultured in vitro and treated with ox-LDL, melatonin, and PD98059 (a selective inhibitor of ERK), respectively. The expression, transcription, and activity of MLCK were measured by western blot, immunohistochemistry, reverse transcription-polymerase chain reaction and gamma-(32)P-adenosine triphosphate (ATP) incorporation, respectively. The results showed that the expression and activity of MLCK were increased in ox-LDL-treated HUVECs and this was decreased by melatonin and PD98059. The expression and activity of MLCK induced by ox-LDL was associated with the phosphorylation of ERK. The...Continue Reading

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Citations

Mar 8, 2013·Molecular and Cellular Biochemistry·Hua WangYuan Wang
Mar 29, 2014·The Journal of Allergy and Clinical Immunology·Nariman A BalengaKirk M Druey
Dec 3, 2013·Molecular and Cellular Endocrinology·Gaia FaveroRita Rezzani
Feb 25, 2017·Molecular Nutrition & Food Research·Robin A WilsonMatthew B Cooke
Jun 29, 2017·Journal of Pineal Research·Almudena CrookeJesús Pintor
Jun 11, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Francesca LuchettiFrancesco Galli
Jul 1, 2020·Hormone Molecular Biology and Clinical Investigation·Yee Lian TiongSoi Moi Chye
Jan 28, 2021·Pharmaceutics·Dalia M Kopustinskiene, Jurga Bernatoniene
May 1, 2021·Seminars in Thrombosis and Hemostasis·Daniel ÁlvarezÁngela P Cadavid J

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