Melatonin relieves sepsis-induced myocardial injury via regulating JAK2/STAT3 signaling pathway

Minerva medica
Genshen ZhenXi Zheng

Abstract

To study the effect of melatonin on myocardial injury of septic rats through regulating the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway. Healthy rats were selected as the samples and divided into blank group, sepsis group and sepsis + melatonin group. The difference in the myocardial tissue structure in the three groups of rats was observed via hematoxylin-eosin (HE) staining, and the messenger ribonucleic acid (mRNA) expression levels of JAK2 and STAT3 in myocardium were compared among groups through fluorescence quantitative polymerase chain reaction (qPCR). Western blotting was applied to detect the protein expressions of JAK2, phosphorylated (p)-JAK2, STAT3 and p-STAT3, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was utilized to determine the cell apoptosis in myocardial tissues. Moreover, the activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) in myocardial tissues as well as the activity of serum creatine kinase (CK) and lactate dehydrogenase (LDH) were measured and compared. There were inflammatory cells in the myocardium in sepsis group, and the pathological changes were milder in sepsis + melatoni...Continue Reading

Citations

Jan 28, 2021·Pharmaceutics·Dalia M Kopustinskiene, Jurga Bernatoniene
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haozhen MaFang Huang

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