Melatonin supplementation during in vitro maturation of oocyte enhances subsequent development of bovine cloned embryos

Journal of Cellular Physiology
Quanli AnJianmin Su

Abstract

Oocyte quality, which is directly related to reprogramming competence, is a major important limiting factor in animal cloning efficiency. Compared with oocytes matured in vivo, in vitro matured oocytes exhibit lower oocyte quality and reprogramming competence primarily because of their higher levels of reactive oxygen species. In this study, we investigate whether supplementing the oocyte maturation medium with melatonin, a free radical scavenger, could improve oocyte quality and reprogramming competence. We found that 10-9 M melatonin effectively alleviated oxidative stress, markedly decreased early apoptosis levels, recovered the integrity of mitochondria, ameliorated the spindle assembly and chromosome alignment in oocytes, and significantly promoted subsequent cloned embryo development in vitro. We also analyzed the effects of melatonin on epigenetic modifications in bovine oocytes. Melatonin increased the global H3K9 acetylation levels, reduced the H3K9 methylation levels, and minimally affected DNA methylation and hydroxymethylation. Genome-wide expression analysis of genes in melatonin-treated and nontreated oocytes was also conducted by high-throughput RNA sequencing. Our results indicated that melatonin ameliorates ooc...Continue Reading

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Citations

May 31, 2020·Reproduction in Domestic Animals = Zuchthygiene·Mansour AboelenainWael A Khalil
May 31, 2019·International Journal of Molecular Sciences·Lei ZhangZekun Guo
Feb 13, 2020·International Journal of Molecular Sciences·Hiroshi TamuraNorihiro Sugino
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Mar 14, 2021·Reproductive Sciences·Zhixin Jiang, Huan Shen
May 11, 2021·Zygote : the Biology of Gametes and Early Embryos·Daohong HeQingshan Gao
Jun 7, 2021·Ageing Research Reviews·Jiska van der ReestPaweł Kordowitzki
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Sep 30, 2021·Zygote : the Biology of Gametes and Early Embryos·Haixia WangEnqi Liu
Nov 13, 2021·Frontiers in Cell and Developmental Biology·Lin MengYunpeng Hou

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