Meloxicam inhibits fipronil-induced apoptosis via modulation of the oxidative stress and inflammatory response in SH-SY5Y cells

Journal of Applied Toxicology : JAT
Jae Hyeon ParkHyun Chul Koh

Abstract

Oxidative stress and inflammatory responses have been identified as key elements of neuronal cell apoptosis. In this study, we investigated the mechanisms by which inflammatory responses contribute to apoptosis in human neuroblastoma SH-SY5Y cells treated with fipronil (FPN). Based on the cytotoxic mechanism of FPN, we examined the neuroprotective effects of meloxicam against FPN-induced neuronal cell death. Treatment of SH-SY5Y cells with FPN induced apoptosis via activation of caspase-9 and -3, leading to nuclear condensation. In addition, FPN induced oxidative stress and increased expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α) via inflammatory stimulation. Pretreatment of cells with meloxicam enhanced the viability of FPN-exposed cells through attenuation of oxidative stress and inflammatory response. FPN activated mitogen activated protein kinase (MAPK) and inhibitors of MAPK abolished FPN-induced COX-2 expression. Meloxicam also attenuated FPN-induced cell death by reducing MAPK-mediated pro-inflammatory factors. Furthermore, we observed both nuclear accumulation of p53 and enhanced levels of cytosolic p53 in a concentration-dependent manner after FPN treatment. Pretreatment of cells with meloxi...Continue Reading

References

Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·S HunotE C Hirsch
Oct 21, 1999·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·A M AghaH Al-Zuhair
Jan 3, 2001·The Journal of Clinical Investigation·P P Tak, G S Firestein
Jun 21, 2001·Hypertension Research : Official Journal of the Japanese Society of Hypertension·M KyawT Tamaki
Oct 5, 2001·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·B S BaekH Y Chung
Jan 12, 2002·The American Journal of Pathology·Yuji YamanishiGary S Firestein
Feb 18, 2003·Neurochemical Research·Richard S MorrisonGwenn A Garden
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Peter TeismannSerge Przedborski
Aug 5, 2003·BioFactors·Martine Torres, Henry Jay Forman
Dec 4, 2003·Mutation Research·João Lúcio Azevedo, Welington Luiz Araujo
May 4, 2004·Annals of Neurology·Yasuhiro ManabeCostantino Iadecola
Feb 15, 2005·Biochemical and Biophysical Research Communications·Eun-Mi ChoiJeong A Han
Nov 1, 2005·Mediators of Inflammation·Riina NieminenEeva Moilanen
Sep 22, 2006·Antioxidants & Redox Signaling·James A McCubreyRichard A Franklin
Nov 7, 2006·Chemico-biological Interactions·Parikshit C DasRandy L Rose
Apr 28, 2007·European Journal of Pharmacology·Hossam M M ArafaFarid M A Hamada
Jun 15, 2007·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Jay P OvermyerMarsha C Black
Jun 6, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Cyril VidauLuc P Belzunces
Jun 11, 2009·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zhixiong ChenQiong Huang
May 19, 2010·Nature Chemical Biology·Chu Chen
Apr 5, 2011·Nature Reviews. Immunology·Olive Leavy

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Citations

Mar 6, 2018·Journal of Alzheimer's Disease : JAD·Morgane CamLaurent Meijer
Nov 22, 2017·Cellular and Molecular Neurobiology·Yazhou XuTao Pang
Feb 8, 2018·Neurochemical Research·Marcos Roberto de OliveiraCristina Ribas Fürstenau
Sep 20, 2016·Critical Reviews in Toxicology·Xu WangZonghui Yuan
Jul 12, 2020·Environmental Science and Pollution Research International·A A KhalafMostafa M Afify
Jun 27, 2019·Apoptosis : an International Journal on Programmed Cell Death·Wenjun ZhouXiang-Shun Cui
Aug 14, 2020·International Journal of Molecular Sciences·Rahul BharatiyaPhilippe De Deurwaerdere
Aug 11, 2020·Environmental Science and Pollution Research International·Mabrouk Attia Abd Abd EldaimNermeen Borai El-Borai
Jan 23, 2021·Environmental Science and Pollution Research International·Shimaa M Abou-ZeidHuda O AbuBakr
Dec 29, 2020·Bioorganic & Medicinal Chemistry Letters·Gabriel MengheresKarl Hemming
Mar 23, 2017·Biochemical and Biophysical Research Communications·Theetat RuangjaroonN Monique Paricharttanakul
Aug 28, 2021·Ecotoxicology and Environmental Safety·Xiao SongJing Qiu

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis