Melting of highly oriented fiber DNA subjected to osmotic pressure

The Journal of Physical Chemistry. B
Andrew WildesMichel Peyrard

Abstract

A pilot study of the possibility to investigate temperature-dependent neutron scattering from fiber-DNA in solution is presented. The study aims to establish the feasibility of experiments to probe the influence of spatial confinement on the structural correlation and the formation of denatured bubbles in DNA during the melting transition. Calorimetry and neutron scattering experiments on fiber samples immersed in solutions of poly(ethylene glycol) (PEG) prove that the melting transition occurs in these samples, that the transition is reversible to some degree, and that the transition is broader in temperature than for humidified fiber samples. The PEG solutions apply an osmotic pressure that maintains the fiber orientation, establishing the feasibility of future scattering experiments to study the melting transition in these samples.

References

Jan 1, 1966·Acta Chemica Scandinavica·A Rupprecht
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D C RauV A Parsegian
Dec 1, 1995·Biophysical Chemistry·R PodgornikV A Parsegian
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·R PodgornikV A Parsegian
Jan 1, 1993·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·T DauxoisA R Bishop
Jun 5, 1989·Physical Review Letters·M Peyrard, A R Bishop
Jul 21, 2006·The Journal of Physical Chemistry. B·A G Cherstvy, A A Kornyshev
Aug 22, 2006·Biophysical Chemistry·Charles H SpinkJonathan B Chaires
Jul 27, 2010·Chemical Communications : Chem Comm·Regina Politi, Daniel Harries
Mar 17, 2011·Physical Review Letters·Andrew WildesMichel Peyrard
Jul 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·D B KnowlesM Thomas Record
Jul 30, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Andrew WildesMichel Peyrard
Mar 22, 2012·The Journal of Physical Chemistry. B·Jessica Valle-OreroMichel Peyrard
Jun 28, 2012·Biophysical Journal·Shu-ichi NakanoNaoki Sugimoto
Sep 26, 2012·Physical Review Letters·Artem BadasyanVladimir Morozov
Jan 22, 2013·The Journal of Physical Chemistry. B·Jessica Valle-OreroMichel Peyrard
Mar 19, 2014·The Journal of Physical Chemistry. B·Federico SebastianiAlessandro Paciaroni

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Citations

Feb 19, 2021·Biopolymers·Adrián GonzálezMichel Peyrard

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