Members of Prevotella Genus Distinctively Modulate Innate Immune and Barrier Functions in a Human Three-Dimensional Endometrial Epithelial Cell Model.

The Journal of Infectious Diseases
Zehra Esra IlhanMelissa M Herbst-Kralovetz

Abstract

Prevotella species are commonly isolated from the reproductive tract of women with obstetric/gynecologic health complications. However, contributions of this genus to changes in local microenvironment are not well characterized. Our objective was to evaluate species-specific effects of Prevotella on the human endometrial epithelium. Thirteen Prevotella strains, originally isolated from the human oral cavity, amniotic fluid, endometrium, or vagina (including women with bacterial vaginosis), were obtained from BEI and ATCC resources. Bacteria were evaluated in silico and in vitro using human endometrial epithelial cells (EEC) grown as monolayers or a 3-dimensional (3D) model. Genomic characterization illustrated metabolic and phylogenetic diversity of Prevotella genus. Among tested species, P. disiens exhibited cytotoxicity. Scanning electron microscopy analysis of the 3D EEC model revealed species-specific colonization patterns and alterations of ultracellular structures. Infection with sialidase-producing P. timonensis resulted in elongated microvilli, and increased MUC3 and MUC4 expression. Infections with Prevotella species, including P. bivia, did not result in significant proinflammatory activation of EEC. Collectively, fin...Continue Reading

References

Dec 15, 1972·American Journal of Obstetrics and Gynecology·H KuramotoY Notake
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R L Sweet
Jun 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S L HillierD A Eschenbach
Aug 17, 1999·Infectious Diseases in Obstetrics and Gynecology·H MikamoT Tamaya
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Dec 31, 2005·Nucleic Acids Research·Ron CaspiPeter D Karp
Dec 17, 2008·Mucosal Immunology·S K LindenM A McGuckin
Dec 17, 2009·Biology of Reproduction·Brooke E HjelmMelissa M Herbst-Kralovetz
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jacques RavelLarry J Forney
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Carlotta De FilippoPaolo Lionetti
Nov 9, 2010·American Journal of Obstetrics and Gynecology·Sabina Cauci, Jennifer Flatow Culhane
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Jan 16, 2013·PloS One·Alexander SwidsinskiZaher Halwani
Dec 20, 2014·American Journal of Obstetrics and Gynecology·Caroline M MitchellDavid Eschenbach
Feb 11, 2016·Biochemical Society Transactions·Nathalie JugeC David Owen
Jun 27, 2018·Pediatric Pulmonology·Maria Di CiccoDiego Peroni
Mar 12, 2019·BJOG : an International Journal of Obstetrics and Gynaecology·R G BrownP R Bennett
Dec 4, 2019·Current Opinion in Infectious Diseases·Christina A MuznyMelissa M Herbst-Kralovetz

❮ Previous
Next ❯

Citations

Dec 22, 2020·IScience·Barath UdayasuryanScott S Verbridge
Apr 17, 2021·Phytotherapy Research : PTR·Haiyue ZhaoLan Shen
Oct 5, 2021·Frontiers in Cellular and Infection Microbiology·Tian LiangLongli Kang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.