PMID: 8995626Feb 1, 1997Paper

Members of the nuclear receptor superfamily regulate transcription from the hepatitis B virus nucleocapsid promoter

Journal of Virology
A K RaneyA McLachlan

Abstract

The role of members of the nuclear receptor superfamily of transcription factors in regulating hepatitis B virus (HBV) transcription was investigated. Hepatocyte nuclear factor 4 (HNF4), the retinoid X receptor (RXR), and the peroxisome proliferator-activated receptor (PPAR) were examined for their capacity to modulate the level of transcriptional activity from the four HBV promoters by transient-transfection analysis in the dedifferentiated hepatoma cell line, HepG2.1. It was found that the nucleocapsid and large surface antigen promoters were transactivated in the presence of HNF4 whereas the enhancer I/X gene, nucleocapsid, and large surface antigen promoters were transactivated in the presence of RXR and PPAR. Characterization of the nuclear receptors interacting with the nucleocapsid promoter region demonstrated that HNF4 is the primary transcription factor binding to the regulatory region spanning nucleotides -127 to -102 whereas HNF4, RXR-PPAR heterodimers, COUPTF1, and ARP1 bind the regulatory region spanning nucleotides -34 to -7. Transcriptional transactivation from the nucleocapsid promoter by HNF4 appears to be mediated through the two HNF4 binding sites in the promoter, whereas modulation of the level of transcript...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S A KliewerR M Evans
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H Su, J K Yee
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B Huan, A Siddiqui
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M López-CabreraA Siddiqui
Nov 1, 1988·Journal of Virology·S Kaneko, R H Miller
Feb 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T S SuF K P'eng
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K ArakiK Yamamura
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K YaginumaK Koike
Jan 1, 1987·Annual Review of Biochemistry·D Ganem, H E Varmus
Nov 6, 1986·The New England Journal of Medicine·O YokosukaK Okuda
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Sep 1, 1984·Molecular and Cellular Biology·J SorgeA E Cutting
Sep 22, 1983·Nature·R CattaneoH Schaller
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M F DuboisP Tiollais
Jul 7, 1995·The Journal of Biological Chemistry·C N PalmerE F Johnson
Oct 1, 1995·Journal of Virology·L G GuidottiF V Chisari
Oct 1, 1994·The Journal of General Virology·A K RaneyA McLachlan

❮ Previous
Next ❯

Citations

Jan 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Thomas F BaumertFritz von Weizsäcker
Sep 30, 2006·World Journal of Gastroenterology : WJG·Silke F FischerStephan Schaefer
Oct 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Amir ShlomaiYosef Shaul
Nov 26, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marianna HöselUlrike Protzer
Jun 16, 2010·Journal of Viral Hepatitis·M Quasdorff, U Protzer
Feb 22, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Fang HeHong Tang
Sep 10, 2016·World Journal of Gastroenterology : WJG·Doo Hyun KimKyun-Hwan Kim
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Susan L UprichardFrancis V Chisari
Jan 11, 2003·The Journal of Biological Chemistry·Wen-Jye LinChawnshang Chang
Mar 18, 2008·The Journal of General Virology·Saffie JammehPeter Karayiannis
Apr 6, 2012·PloS One·Wei HuShengqi Wang
Jan 23, 2016·Journal of Virology·Takahisa KouwakiYoshiharu Matsuura
Sep 13, 2002·Journal of Viral Hepatitis·Naazneen MoollaPatrick Arbuthnot
Apr 15, 2009·PPAR Research·Laurent DubuquoySébastien Dharancy
Jul 25, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Tzu-Jon LinHan-Jung Lee
Feb 15, 2005·Journal of Virology·Myrna M MillerKarel A Schat
Sep 5, 2008·Journal of Virology·Christophe RamièrePatrice André

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.