Membrane-anchored cytochrome cy mediated microsecond time range electron transfer from the cytochrome bc1 complex to the reaction center in Rhodobacter capsulatus

Biochemistry
H MyllykallioFevzi Daldal

Abstract

In Rhodobacter capsulatus, the soluble cytochrome (cyt) c2 and membrane-associated cyt cy are the only electron carriers which operate between the photochemical reaction center (RC) and the cyt bc1 complex. In this work, cyt cy mediated microsecond time range electron transfer kinetics were studied by light-activated time-resolved absorption spectroscopy using a mutant strain lacking cyt c2. In intact cells and in isolated chromatophores of this mutant, only approximately 30% of the RCs had their photooxidized primary donor rapidly rereduced by cyt cy. Of these 30%, about half were reduced with a half-time of approximately 5 micros attributed to preformed complexes, and the other half with a half-time of approximately 40 micros attributed to cyt cy having to move from another site. This slower phase was affected by addition of glycerol, indicating its dependence on the viscosity of the medium. Cyt cy, despite its rereduction by ubihydroquinone oxidation in the millisecond time range, remained virtually unable to deliver electrons to other RCs which stayed photooxidized for several seconds. Furthermore, using two flashes separated by a variable time interval, it was shown that the fast electron donating complex was reformed in a...Continue Reading

References

Dec 17, 1987·Biochimica Et Biophysica Acta·R C Prince, F Daldal
Jun 1, 1960·Journal of General Microbiology·W R SISTROM

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Citations

Dec 29, 2000·Trends in Microbiology·H MyllykallioF Daldal
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·H MyllykallioF Daldal
Jul 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Camilla Rydström LundinPeter Brzezinski
Sep 30, 2016·Scientific Reports·Simone GrafPeter Brzezinski
Mar 26, 2003·FEBS Letters·Frauke BaymannFraser A Armstrong
Sep 7, 2020·Cell Death & Disease·Alexandra TothMartin Ott
Mar 24, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Alexei StuchebrukhovPeter Brzezinski
Apr 29, 2009·Biochemistry·Marina KonortyAbraham Minsky
Jun 3, 2021·Membranes·Deborah M BoesDuncan G G McMillan
Jun 30, 2021·Chemical Reviews·Peter BrzezinskiPia Ädelroth

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