Membrane-anchored incorporation of a foreign protein in recombinant influenza virions

Virology
Y ZhouE Neumeier

Abstract

The RNA polymerase I system for in vivo synthesis of recombinant influenza vRNA molecules was used for the expression of a chimeric protein, consisting of the 341-amino-acid ectodomain of the glycoprotein E2 of classical swine fever virus and the 37-amino-acid C-terminal membrane anchor of the influenza virus hemagglutinin (HA). During infection with an influenza A helper virus the amplified pseudo-viral RNA was packaged into progeny virions together with influenza vRNA segments. The foreign fusion protein E2-HA was shown to be physically incorporated into the viral envelope. Incorporation of a third major glycoprotein into the envelope did not affect biological functions of HA and neuraminidase that are required for the generation of infectious virus particles. Based on mutational analyses of the cytoplasmic tail of E2-HA fusion proteins three modes of interaction during virus budding have been observed: nonspecific low-level incorporation (truncated tails), specific full-level incorporation (wild-type amino acid sequence or minor variations of it), and exclusion from incorporation (elongated tails).

References

Nov 1, 1991·Virology·M EnamiP Palese
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Mar 1, 1980·Journal of Virology·J K McDougallD A Galloway
Jul 1, 1994·Journal of Virology·N PercyP Palese
Feb 11, 1994·Science·M ChalfieD C Prasher
Aug 11, 1993·Nucleic Acids Research·A ZobelG Hobom
Dec 1, 1996·Current Biology : CB·K R SiemeringJ Haseloff
Jul 1, 1995·The Journal of General Virology·G Neumann, G Hobom

❮ Previous
Next ❯

Citations

Jul 17, 2001·RNA·S TchatalbachevG Hobom
Dec 26, 2001·Journal of Virology·Tokiko WatanabeYoshihiro Kawaoka
May 14, 2004·Journal of Virology·Masato HattaYoshihiro Kawaoka
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yutaka FujiiYoshihiro Kawaoka
May 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·E HoffmannR G Webster
Jan 12, 2002·Reviews in Medical Virology·Gabriele Neumann, Yoshihiro Kawaoka
Jul 26, 2011·Journal of Structural Biology·Paul GuichardSergio Marco
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Sep 5, 2001·Virology·G Neumann, Y Kawaoka
Apr 24, 2004·The Journal of General Virology·Wiebke KöhlGeorg Herrler
Nov 26, 1999·The Journal of General Virology·R Flick, G Hobom
Jul 10, 1999·Journal of Virology·M FlintJ A McKeating

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.