Membrane and firing properties of glutamatergic and GABAergic neurons in the rat medial vestibular nucleus

Journal of Neurophysiology
Tomonori TakazawaSeiji Ozawa

Abstract

In previous studies, neurons in the medial vestibular nucleus (MVN) were classified mainly into 2 types according to their intrinsic membrane properties in in vitro slice preparations. However, it has not been determined whether the classified neurons are excitatory or inhibitory ones. In the present study, to clarify the relationship between the chemical and electrophysiological properties of MVN neurons, we explored mRNAs of cellular markers for GABAergic (glutamic acid decarboxylase 65, 67, and neuronal GABA transporter), glutamatergic (vesicular glutamate transporter 1 and 2), glycinergic (glycine transporter 2), and cholinergic neurons (choline acetyltransferase and vesicular acetylcholine transporter) expressed in electrophysiologically characterized MVN neurons in rat brain stem slice preparations. For this purpose, we combined whole cell patch-clamp recording analysis with single-cell reverse transcription-polymerase chain reaction (RT-PCR) analysis. We examined the membrane properties such as afterhyperpolarization (AHP), firing pattern, and response to hyperpolarizing current pulse to classify MVN neurons. From the single-cell RT-PCR analysis, we found that GABAergic neurons consisted of heterogeneous populations with...Continue Reading

References

Jan 1, 1979·Annual Review of Neuroscience·W Precht
Mar 15, 1976·Experimental Brain Research·A W Louie, J Kimm
May 22, 1992·Annals of the New York Academy of Sciences·N FuruyaT Koizumi
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Q R LiuN Nelson
Jul 19, 1991·Brain Research·G A Kevetter, R D Hoffman
Jan 1, 1991·Experimental Brain Research·M SerafinM Mühlethaler
Jul 1, 1991·Neuron·M G ErlanderA J Tobin
Jan 1, 1974·The Journal of Membrane Biology·S Hagiwara, K Takahashi
Jan 1, 1972·Experimental Brain Research·R BakerR Llinás
Nov 1, 1965·Journal of Neurophysiology·H Shimazu, W Precht
Dec 1, 1980·Journal of Neuroscience Methods·G PaxinosP C Emson
Oct 28, 1982·Brain Research·J V Halliwell, P R Adams
May 1, 1980·Pflügers Archiv : European journal of physiology·K Yanagihara, H Irisawa
Jan 1, 1981·Neuroradiology·K WesterE Hauglie-Hanssen
May 1, 1995·Trends in Neurosciences·T B UsdinJ D Erickson
Apr 1, 1995·Journal of Neurophysiology·J S Stahl, J I Simpson
Jan 1, 1995·Brain Research. Brain Research Reviews·C de WaeleP P Vidal
May 1, 1995·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·S du Lac, S G Lisberger
Mar 1, 1994·The European Journal of Neuroscience·H Drouin, A Hermann
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·B NiS M Paul

❮ Previous
Next ❯

Citations

Feb 19, 2011·Experimental Brain Research·Daniel EugènePierre-Paul Vidal
Jul 18, 2012·PloS One·Tomonori TakazawaAmy B Macdermott
Jun 8, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Takashi KodamaSascha du Lac
Nov 18, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kristine E KolkmanSascha du Lac
May 18, 2013·Brain Structure & Function·Eva RáczClara Matesz
May 1, 2008·The European Journal of Neuroscience·Masato ShinoYasuhiko Saito
Jul 18, 2006·Current Opinion in Neurobiology·Aryn H Gittis, Sascha du Lac
Feb 16, 2006·Neurobiology of Disease·S GigoutM Avoli
Jun 2, 2005·Trends in Neurosciences·Rafael Gutiérrez
Jan 15, 2013·Neuroscience Letters·Chang-Hee KimSang Jeong Kim
Aug 28, 2007·Neuroscience Research·Yasuhiko Saito, Seiji Ozawa
Dec 31, 2005·Journal of Neurophysiology·Ruba Benini, Massimo Avoli
Sep 5, 2008·Journal of Neurophysiology·Michael L MolineuxRay W Turner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.